Novel Lipopeptides, Kiostostatins A-E from a Marine-Derived Bacterium Bacillus subtilis

Title
Novel Lipopeptides, Kiostostatins A-E from a Marine-Derived Bacterium Bacillus subtilis
Author(s)
TAREQ; 김지혜; 이민아; 이희승; 이종석; 이연주; 신희재
KIOST Author(s)
Lee, Min Ah(이민아)Lee, Hyi Seung(이희승)Lee, Jong Seok(이종석)Lee, Yeon Ju(이연주)Shin, Hee Jae(신희재)
Alternative Author(s)
TAREQ; 김지혜; 이민아; 이희승; 이종석; 이연주; 신희재
Publication Year
2013-11-12
Abstract
As part of our continuing interest to discover secondary metabolites from marine sponge and sediment derived bacteria, a bacterial strain 109GGC020 showing good antimicrobial activity was isolated from a marine sediment sample collected from Gageocho, Republic of Korea. The bacterium was identified by 16s rRNA sequence analysis as Bacillus subtilis. After 7 days fermentation of the strain in optimum growth condition, five linear lipopeptides, kiostostatins A-E (1-5), were isolated using chromatographic procedures. These lipopeptides were characterized by extensive 1D, 2D and high resolution ESIMS data analysis and their stereoconfigurations were assigned by chemical derivatization studies. The minimum inhibitory concentrations (MICs) of kiostostatins 1-5 were evaluated against bacteria (B. subtilis, S. aeureus, S. typhi and P. aeruginosa) and fungi (R. solani, B. cinerea and C. acutatum) by broth dilution assay. Kiostostatins exhibited antibacterial and antifungal activity with MICs values of 0.003-0.022 μM. These results demonstrated 1-5 as potent antibiotics and fungicides. Furthermore, kiostostatins 1-5 displayed cytotoxicity against six human cancer cell lines with GI50 values of 0.01-0.04 μM.m Gageocho, Republic of Korea. The bacterium was identified by 16s rRNA sequence analysis as Bacillus subtilis. After 7 days fermentation of the strain in optimum growth condition, five linear lipopeptides, kiostostatins A-E (1-5), were isolated using chromatographic procedures. These lipopeptides were characterized by extensive 1D, 2D and high resolution ESIMS data analysis and their stereoconfigurations were assigned by chemical derivatization studies. The minimum inhibitory concentrations (MICs) of kiostostatins 1-5 were evaluated against bacteria (B. subtilis, S. aeureus, S. typhi and P. aeruginosa) and fungi (R. solani, B. cinerea and C. acutatum) by broth dilution assay. Kiostostatins exhibited antibacterial and antifungal activity with MICs values of 0.003-0.022 μM. These results demonstrated 1-5 as potent antibiotics and fungicides. Furthermore, kiostostatins 1-5 displayed cytotoxicity against six human cancer cell lines with GI50 values of 0.01-0.04 μM.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26553
Bibliographic Citation
International Marine Biotechnology Conference, pp.155, 2013
Publisher
International
Type
Conference
Language
English
Publisher
International
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