Evaluation of anti-inflammatory activity of marine algae in LPS-stimulated RAW 264.7 cells
DC Field | Value | Language |
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dc.contributor.author | 허수진 | - |
dc.contributor.author | 예보람 | - |
dc.contributor.author | 장지이 | - |
dc.contributor.author | 김민선 | - |
dc.contributor.author | 김준성 | - |
dc.contributor.author | 정원교 | - |
dc.contributor.author | 오철홍 | - |
dc.contributor.author | 강도형 | - |
dc.date.accessioned | 2020-07-16T06:50:53Z | - |
dc.date.available | 2020-07-16T06:50:53Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2013-11-13 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/26551 | - |
dc.description.abstract | Inflammation is complex process involving a variety of immune cells that defend the body from harmful stimuli. However, pro-inflammatory cytokines and inflammatory mediators can also exacerbate diseases such as cancer. The aim of this study was to identify a natural effective remedy for inflammation. We isolated a functional algal compound from marine algae and identified as a kinds of chromene, sargachromanol G (SG). In this study, the anti-inflammatory effect and the action mechanism of SG have been investigated in murine macrophage cell line RAW 264.7. SG dose-dependently inhibited the production of inflammatory markers [nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), and cyclooxygenase-2 (COX-2)] and pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6] induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of SG, we studied LPS induced nuclear factor-κB (NF-κB) activation and mitogen-activated protein kinases (MAPKs) phosphorylation. SG inhibited the phosphorylation IκB-α and NF-κB (p65 and p50) and MAPK (ERK1/2, JNK, and p38) in a dose dependent manner. These results suggest that the anti-inflammatory activity of SG results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-κB activation and MAPK phosphorylation. was to identify a natural effective remedy for inflammation. We isolated a functional algal compound from marine algae and identified as a kinds of chromene, sargachromanol G (SG). In this study, the anti-inflammatory effect and the action mechanism of SG have been investigated in murine macrophage cell line RAW 264.7. SG dose-dependently inhibited the production of inflammatory markers [nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), and cyclooxygenase-2 (COX-2)] and pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6] induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of SG, we studied LPS induced nuclear factor-κB (NF-κB) activation and mitogen-activated protein kinases (MAPKs) phosphorylation. SG inhibited the phosphorylation IκB-α and NF-κB (p65 and p50) and MAPK (ERK1/2, JNK, and p38) in a dose dependent manner. These results suggest that the anti-inflammatory activity of SG results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-κB activation and MAPK phosphorylation. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | International Marine Biotechnology Conference | - |
dc.relation.isPartOf | 10th International Mairne Biotechnology Conference | - |
dc.title | Evaluation of anti-inflammatory activity of marine algae in LPS-stimulated RAW 264.7 cells | - |
dc.type | Conference | - |
dc.citation.endPage | 92 | - |
dc.citation.startPage | 92 | - |
dc.citation.title | 10th International Mairne Biotechnology Conference | - |
dc.contributor.alternativeName | 허수진 | - |
dc.contributor.alternativeName | 예보람 | - |
dc.contributor.alternativeName | 장지이 | - |
dc.contributor.alternativeName | 김민선 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | 10th International Mairne Biotechnology Conference, pp.92 | - |
dc.description.journalClass | 1 | - |