Evaluation of anti-inflammatory activity of marine algae in LPS-stimulated RAW 264.7 cells

DC Field Value Language
dc.contributor.author 허수진 -
dc.contributor.author 예보람 -
dc.contributor.author 장지이 -
dc.contributor.author 김민선 -
dc.contributor.author 김준성 -
dc.contributor.author 정원교 -
dc.contributor.author 오철홍 -
dc.contributor.author 강도형 -
dc.date.accessioned 2020-07-16T06:50:53Z -
dc.date.available 2020-07-16T06:50:53Z -
dc.date.created 2020-02-11 -
dc.date.issued 2013-11-13 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/26551 -
dc.description.abstract Inflammation is complex process involving a variety of immune cells that defend the body from harmful stimuli. However, pro-inflammatory cytokines and inflammatory mediators can also exacerbate diseases such as cancer. The aim of this study was to identify a natural effective remedy for inflammation. We isolated a functional algal compound from marine algae and identified as a kinds of chromene, sargachromanol G (SG). In this study, the anti-inflammatory effect and the action mechanism of SG have been investigated in murine macrophage cell line RAW 264.7. SG dose-dependently inhibited the production of inflammatory markers [nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), and cyclooxygenase-2 (COX-2)] and pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6] induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of SG, we studied LPS induced nuclear factor-κB (NF-κB) activation and mitogen-activated protein kinases (MAPKs) phosphorylation. SG inhibited the phosphorylation IκB-α and NF-κB (p65 and p50) and MAPK (ERK1/2, JNK, and p38) in a dose dependent manner. These results suggest that the anti-inflammatory activity of SG results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-κB activation and MAPK phosphorylation. was to identify a natural effective remedy for inflammation. We isolated a functional algal compound from marine algae and identified as a kinds of chromene, sargachromanol G (SG). In this study, the anti-inflammatory effect and the action mechanism of SG have been investigated in murine macrophage cell line RAW 264.7. SG dose-dependently inhibited the production of inflammatory markers [nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), and cyclooxygenase-2 (COX-2)] and pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6] induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of SG, we studied LPS induced nuclear factor-κB (NF-κB) activation and mitogen-activated protein kinases (MAPKs) phosphorylation. SG inhibited the phosphorylation IκB-α and NF-κB (p65 and p50) and MAPK (ERK1/2, JNK, and p38) in a dose dependent manner. These results suggest that the anti-inflammatory activity of SG results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-κB activation and MAPK phosphorylation. -
dc.description.uri 1 -
dc.language English -
dc.publisher International Marine Biotechnology Conference -
dc.relation.isPartOf 10th International Mairne Biotechnology Conference -
dc.title Evaluation of anti-inflammatory activity of marine algae in LPS-stimulated RAW 264.7 cells -
dc.type Conference -
dc.citation.endPage 92 -
dc.citation.startPage 92 -
dc.citation.title 10th International Mairne Biotechnology Conference -
dc.contributor.alternativeName 허수진 -
dc.contributor.alternativeName 예보람 -
dc.contributor.alternativeName 장지이 -
dc.contributor.alternativeName 김민선 -
dc.contributor.alternativeName 오철홍 -
dc.contributor.alternativeName 강도형 -
dc.identifier.bibliographicCitation 10th International Mairne Biotechnology Conference, pp.92 -
dc.description.journalClass 1 -
Appears in Collections:
Jeju Research Institute > Jeju Marine Research Center > 2. Conference Papers
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse