Development of Monitoring and Detection Technologies for Marine Pathogens

Abstract

Marine pathogens have been regarded as the main culprit causing prevalent and virulent disease associated with marine life. Despite decades of intense study of the effect of marine pathogens on the disease outbreaks, the fundamental reasons for those are still poorly described. Even less well known are the dynamics of intermittent, ephemeral, threshold phenomena such as disease outbreaks. Furthermore, marine pathogens have more rapid generation times, allowing to them to adapt more quickly to changing environments and potentially to be more widespread with out of control pathogens. In addition, the inability even to indentify most causative agents and the lack of basic epidemiological data for diseased populations limit our ability to examine host-pathogen interaction. Therefore, unlike the well established pathogen-controlling system on land, the management of disease breaks in marine environment has been considered as virtually impossible mission. Given this prospect, there is an urgent need for interdisciplinary and systematical studies of marine disease, understanding mechanisms of disease resistance in marine organisms. In this study, we focused on the development of molecular tools to detect and monitor pathogens as marine pathogen diagnosis and surveillance system. Firstly, using next generation sequencing, the diversity and composition of pathogens in waster masses was indentified. Secondly, the new molecular for those are still poorly described. Even less well known are the dynamics of intermittent, ephemeral, threshold phenomena such as disease outbreaks. Furthermore, marine pathogens have more rapid generation times, allowing to them to adapt more quickly to changing environments and potentially to be more widespread with out of control pathogens. In addition, the inability even to indentify most causative agents and the lack of basic epidemiological data for diseased populations limit our ability to examine host-pathogen interaction. Therefore, unlike the well established pathogen-controlling system on land, the management of disease breaks in marine environment has been considered as virtually impossible mission. Given this prospect, there is an urgent need for interdisciplinary and systematical studies of marine disease, understanding mechanisms of disease resistance in marine organisms. In this study, we focused on the development of molecular tools to detect and monitor pathogens as marine pathogen diagnosis and surveillance system. Firstly, using next generation sequencing, the diversity and composition of pathogens in waster masses was indentified. Secondly, the new molecular