Inhibition of lipopolysaccharide-stimulated inflammatory mediators by chromene in RAW 264.7 cells
DC Field | Value | Language |
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dc.contributor.author | 장지이 | - |
dc.contributor.author | 김준성 | - |
dc.contributor.author | 예보람 | - |
dc.contributor.author | 김민선 | - |
dc.contributor.author | 이아름 | - |
dc.contributor.author | 윤원종 | - |
dc.contributor.author | 김길남 | - |
dc.contributor.author | 오철홍 | - |
dc.contributor.author | 강도형 | - |
dc.contributor.author | 허수진 | - |
dc.date.accessioned | 2020-07-16T05:51:29Z | - |
dc.date.available | 2020-07-16T05:51:29Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2014-04-17 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/26375 | - |
dc.description.abstract | Inflammation is complex process involving a variety of immune cells that defend the body from harmful stimuli. However, pro-inflammatory cytokines and inflammatory mediators can also exacerbate diseases such as cancer. The aim of this study was to identify a natural effective remedy for inflammation. We isolated a functional algal chromene compound from Sargassum siliquastrum, named sargachromanol D (SD). We evaluated the anti-inflammatory effect of SD on lipopolysaccharide (LPS)-exposed RAW 264.7 cells by measuring cell viability, cytotoxicity, and production of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL-1β, and IL-6). SD inhibited production of NO and PGE2 from LPS-induced cells by preventing the expression of inflammatory mediators such as iNOS and COX-2 in a dose-dependent manner. Concurrently, levels of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 were reduced with increasing concentrations of SD. In addition, SD inhibited the activation of NF-κB and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner. These results indicate that SD inhibits LPS-stimulated inflammation by inhibition of the NF-κB and MAPKs pathways in macrophages. was to identify a natural effective remedy for inflammation. We isolated a functional algal chromene compound from Sargassum siliquastrum, named sargachromanol D (SD). We evaluated the anti-inflammatory effect of SD on lipopolysaccharide (LPS)-exposed RAW 264.7 cells by measuring cell viability, cytotoxicity, and production of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL-1β, and IL-6). SD inhibited production of NO and PGE2 from LPS-induced cells by preventing the expression of inflammatory mediators such as iNOS and COX-2 in a dose-dependent manner. Concurrently, levels of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 were reduced with increasing concentrations of SD. In addition, SD inhibited the activation of NF-κB and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner. These results indicate that SD inhibits LPS-stimulated inflammation by inhibition of the NF-κB and MAPKs pathways in macrophages. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | 한국조류학회 | - |
dc.relation.isPartOf | 2014 Wando International Marine Algal Symposium | - |
dc.title | Inhibition of lipopolysaccharide-stimulated inflammatory mediators by chromene in RAW 264.7 cells | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 203 | - |
dc.citation.startPage | 203 | - |
dc.citation.title | 2014 Wando International Marine Algal Symposium | - |
dc.contributor.alternativeName | 장지이 | - |
dc.contributor.alternativeName | 예보람 | - |
dc.contributor.alternativeName | 김민선 | - |
dc.contributor.alternativeName | 이아름 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.contributor.alternativeName | 허수진 | - |
dc.identifier.bibliographicCitation | 2014 Wando International Marine Algal Symposium, pp.203 | - |
dc.description.journalClass | 1 | - |