Comparative Proteomic Analysis of Extracellular vesicles (EVs) from the Hyperthermophilic archaeon, Thermococcusonnurineus NA1

Abstract

Thermococcus onnurineus NA1 is able to produce extracellular vesicles (ToEVs) under anaerobic cultivation at 80oC. EVs were purified through ultra membrane filtration and CsCl-density gradient ultracentrifugation. Two major bands (B & C) were obtained and theirs proteomes were further analyzed by LC MS/MS spectrometry. It was revealed that the ToEVs of band B and C contained 294 and 426 proteins, respectively. Ratio of membrane protein to total protein in bands B and C ToEVs was slightly higher than that of parental cells. The analysis of predicted metabolic function of proteins from two major bands based on the COG database indicated that there was no big discrepancy between them. Proteomic comparison among parental cell, band B and C ToEVs using Venn diagram showed overlapping zone where we could find 238 proteins. The total numbers of protein which was found from EVs but not from parent cells were 15. The role of specific proteins mainly packaged from parental cell to ToEVs will be discussed.re obtained and theirs proteomes were further analyzed by LC MS/MS spectrometry. It was revealed that the ToEVs of band B and C contained 294 and 426 proteins, respectively. Ratio of membrane protein to total protein in bands B and C ToEVs was slightly higher than that of parental cells. The analysis of predicted metabolic function of proteins from two major bands based on the COG database indicated that there was no big discrepancy between them. Proteomic comparison among parental cell, band B and C ToEVs using Venn diagram showed overlapping zone where we could find 238 proteins. The total numbers of protein which was found from EVs but not from parent cells were 15. The role of specific proteins mainly packaged from parental cell to ToEVs will be discussed.