Neptunomonas daebuensis sp. nov., Isolated from a Sediment of the Daebu-Island, Korea and Emended Description of Members of the Genus Neptunomonas

Abstract

A gram-negative, aerobic, rod-shaped and non-motile marine bacterium, designated as MEBiC 06243T was isolated from a sediment at the Daebu-Island of the Yellow Sea, Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC06243T showed high similarity with the Neptunomonas naphthovorans NAG-2N-126T (96.3 %). Growth was observed at 10.2-38.7 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 7) and with 0-7 % (optimum 2.5) NaCl. The predominant cellular fatty acids were C10:0 3-OH (6.1%), C12:0, (5.8%), C16:0, (30.5%), C18:1ω7c (21.6%) and summed feature 3 (30.7%). The DNA G+C contents is 41.4 mol%. The major respiratory quinone is Q-8. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified lipids, one unidentified amino-phospholipid and three unidentified aminolipids were detected as major polar lipids. On the basis of this polyphasic taxonomic data, strain MEBiC06243T should be classified as a novel species in the genus Neptunomonas and it is proposed as Neptunomonas daebuensis sp. nov. The type strain is MEBiC 06243T (=KCCM 42975T =JCM 18291T). Emended descriptions on the genus Neptunomonas were also given. showed high similarity with the Neptunomonas naphthovorans NAG-2N-126T (96.3 %). Growth was observed at 10.2-38.7 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 7) and with 0-7 % (optimum 2.5) NaCl. The predominant cellular fatty acids were C10:0 3-OH (6.1%), C12:0, (5.8%), C16:0, (30.5%), C18:1ω7c (21.6%) and summed feature 3 (30.7%). The DNA G+C contents is 41.4 mol%. The major respiratory quinone is Q-8. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified lipids, one unidentified amino-phospholipid and three unidentified aminolipids were detected as major polar lipids. On the basis of this polyphasic taxonomic data, strain MEBiC06243T should be classified as a novel species in the genus Neptunomonas and it is proposed as Neptunomonas daebuensis sp. nov. The type strain is MEBiC 06243T (=KCCM 42975T =JCM 18291T). Emended descriptions on the genus Neptunomonas were also given.