Resting cysts, and effects of temperature and salinity on the growth of vegetative cells of the potentially harmful species

Title
Resting cysts, and effects of temperature and salinity on the growth of vegetative cells of the potentially harmful species
Publication Year
2014-05-27
Abstract
The potentially harmful species Alexandrium insuetum established by the incubation of resting cysts isolated from sediment trap samples collected at Jinhae-Masan Bay, Korea was characterized by morphological and phylogenetic analysis. The effects of temperature and salinity on the growth of A. insuetum were also investigated. The resting cysts are characterized by a spherical shape, a small size (20-25 μm) and the presence of either three or four red accumulation bodies. The similarity of morphological features of the resting cysts to those of other species of the minutum group (consisting of Alexandrium minutum and A. tamutum) indicates that the morphological features of resting cysts might improve the accuracy of the grouping of Alexandrium species. A. insuetum germinated from the resting cysts is morphologically consistent with vegetative cells reported from Korean and Japanese coastal areas, and has an partial large subunit (LSU) rDNA sequence identical to that from Japanese strains. The growth of A. insuetum was observed between 20 and 35 psu, with increasing temperature however at 25°C, A. insuetum could grow even at 15 psu. The highest growth rate (0.60 d-1)wasobservedat25°Cand25psu,whichishigherthanthepreviouslyreportedgrowthrateofA. tamarense, which is responsible for outbreaks of paralytic shellfish poisoining and blooms in Jinhae-Masan Bay. These results suggest that the proliferation of A. insuetum inffects of temperature and salinity on the growth of A. insuetum were also investigated. The resting cysts are characterized by a spherical shape, a small size (20-25 μm) and the presence of either three or four red accumulation bodies. The similarity of morphological features of the resting cysts to those of other species of the minutum group (consisting of Alexandrium minutum and A. tamutum) indicates that the morphological features of resting cysts might improve the accuracy of the grouping of Alexandrium species. A. insuetum germinated from the resting cysts is morphologically consistent with vegetative cells reported from Korean and Japanese coastal areas, and has an partial large subunit (LSU) rDNA sequence identical to that from Japanese strains. The growth of A. insuetum was observed between 20 and 35 psu, with increasing temperature however at 25°C, A. insuetum could grow even at 15 psu. The highest growth rate (0.60 d-1)wasobservedat25°Cand25psu,whichishigherthanthepreviouslyreportedgrowthrateofA. tamarense, which is responsible for outbreaks of paralytic shellfish poisoining and blooms in Jinhae-Masan Bay. These results suggest that the proliferation of A. insuetum in
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26196
Bibliographic Citation
2014년도 한국해양과학기술협의회 공동학술대회, pp.175 - 184, 2014
Publisher
한국해양과학기술협의회
Type
Conference
Language
English
Publisher
한국해양과학기술협의회
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