Ochrovirga pacifica gen. nov., sp. nov., a novel agar-lytic marine bacterium of the family Flavobacteriaceae isolated from a seaweed

Abstract

A strain designated as S85T was isolated from a seaweed collected from coastal area of Chuuk State in Micronesia. The strain was gram-negative, rod-shaped, non-motile and formed yellow colonies on the SWY agar (0.2% yeast extract and 1.5% agar in seawater) and Marine agar 2216. The strain grew at pH 5-9 (optimum, pH 8), at 15-40 °C (optimum, 25-28 °C), and with 1-9% (w/v) NaCl (optimum, 3%). The phylogenetic analysis based on 16S rRNA gene sequence showed that strain S85 T was related to Lutibacter litoralis CL-TF09 T and Maritimimonas rapanae A31 T with 91.4% and with 90.5% similarity, respectively. The dominant fatty acids were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C16:0 3-OH and summed feature 3 comprised of C16:1 w7c and/or iso-C15:0 2-OH. The major isoprenoid quinone was MK-6. The DNA G+C content of the type strain was 34.6 mol%. The major polar lipids were phosphatidylethanolamine, an unknown glycolipid and two unknown polar lipids. Based on this polyphasic taxonomic data, strain S85T stands for a novel species of a new genus, and we propose the name Ochrovirga pacifica gen. nov., sp. nov.. The type strain of Ochrovirga pacifica is S85 T (=KCCM 90106T =JCM 18327T39 ).gar in seawater) and Marine agar 2216. The strain grew at pH 5-9 (optimum, pH 8), at 15-40 °C (optimum, 25-28 °C), and with 1-9% (w/v) NaCl (optimum, 3%). The phylogenetic analysis based on 16S rRNA gene sequence showed that strain S85 T was related to Lutibacter litoralis CL-TF09 T and Maritimimonas rapanae A31 T with 91.4% and with 90.5% similarity, respectively. The dominant fatty acids were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C16:0 3-OH and summed feature 3 comprised of C16:1 w7c and/or iso-C15:0 2-OH. The major isoprenoid quinone was MK-6. The DNA G+C content of the type strain was 34.6 mol%. The major polar lipids were phosphatidylethanolamine, an unknown glycolipid and two unknown polar lipids. Based on this polyphasic taxonomic data, strain S85T stands for a novel species of a new genus, and we propose the name Ochrovirga pacifica gen. nov., sp. nov.. The type strain of Ochrovirga pacifica is S85 T (=KCCM 90106T =JCM 18327T39 ).