초고온성 고세균 Thermococcus onnurineus NA1을 이용한 석탄유래 합성가스로부터 바이오수소 생산

Abstract

The hyperthermophilic archaeon, Thermococcus onnurineus NA1 was known for a good bio-hydrogen producer from carbon monoxide (CO) through the water-gas shift (WGS) reaction (CO + H2O → CO2 + H2). Recently, we reported a metabolically engineered T. onnurineus NA1, MC01, which showed the enhanced H2 productivity from CO at least 2-fold higher than wild-type strain. In addition, it was also found that MC01 could produce H2 from off-gases of the steel-mill industry, LDG and BFG, indicating the feasibility of bio-hydrogen production from CO-containing gas of industrial origin.In this study, we investigated H2 production by MC01 from coal-derived syngas which is currently mass-produced through the industrial gasification processes. From a series of batch cultivation using simulated gas of coal-derived syngas, it was confirmed that gas supply rate and agitation speed are main parameters for microbial WGS reaction. The cell growth and H2 production rate (HPR) were linearly correlated with gas flow rate from 138 to 414 mL/min and agitation speed from 300 to 900 rpm. Based on the results, batch culture was conducted at 600 rpm with gas supply rate of 276 mL/min to assess the activity of MC01 for H2 production from real coal-derived syngas. As a result, MC01 was found to have a good activity for H2 production from coal-derived syngas showing the same maximum H2 production rate of 50 mmol/L/h as that obtained from simulated gaered T. onnurineus NA1, MC01, which showed the enhanced H2 productivity from CO at least 2-fold higher than wild-type strain. In addition, it was also found that MC01 could produce H2 from off-gases of the steel-mill industry, LDG and BFG, indicating the feasibility of bio-hydrogen production from CO-containing gas of industrial origin.In this study, we investigated H2 production by MC01 from coal-derived syngas which is currently mass-produced through the industrial gasification processes. From a series of batch cultivation using simulated gas of coal-derived syngas, it was confirmed that gas supply rate and agitation speed are main parameters for microbial WGS reaction. The cell growth and H2 production rate (HPR) were linearly correlated with gas flow rate from 138 to 414 mL/min and agitation speed from 300 to 900 rpm. Based on the results, batch culture was conducted at 600 rpm with gas supply rate of 276 mL/min to assess the activity of MC01 for H2 production from real coal-derived syngas. As a result, MC01 was found to have a good activity for H2 production from coal-derived syngas showing the same maximum H2 production rate of 50 mmol/L/h as that obtained from simulated ga