A Nile Red staining method for microplastic identification and quantification

Title
A Nile Red staining method for microplastic identification and quantification
Author(s)
송영경; 홍상희; 장미; 한기명; 심원준
KIOST Author(s)
Song, Young Kyoung(송영경)Hong, Sang Hee(홍상희)Jang, Mi(장미)Han, Gi Myung(한기명)Shim, Won Joon(심원준)
Publication Year
2014-10-23
Abstract
Microplastics are classically visually identified with a microscope. Microscopic identification has low reliability especially in small (e.g. <200μm) transparent or white particles. FT-IR equipped with a microscope has been frequently applied to spectroscopic identification of micro-scale polymers including qualitative confirmation of polymer types. It is a little hard to detect microplastics less than 50μm in size. We aim to develop a subsidiary method to facilitate identification of synthetic polymers using a fluorescent dye.Nile Red (NR), a fluorescent dye, which was well known to dye neutral lipid in cell and tissue sample is used in this study. It is strongly fluorescent only in the presence of a hydrophobic environment. Applicability of NR for identification and quantification of microplastics was tested and staining condition was optimized in this study. Micro-sized polyethylene (PE), polypropylene (PP) and expanded polystyrene (EPS) were used as model plastics. Stained polymer particles were recognized better in green fluorescence (Ex. 534-558 and Em. 515-565) than red (Ex. 534-558 and Em. >590). The 50mg/L solution showed higher fluorescence than 5 mg/L solution. Fluorescence intensity was in the order of EPS > PP > PE. The developed NR staining method was successfully applied to identify and quantify the fragmented polymer particles in a laboratory accelerated mechanical abrasion study. Other polymeied to spectroscopic identification of micro-scale polymers including qualitative confirmation of polymer types. It is a little hard to detect microplastics less than 50μm in size. We aim to develop a subsidiary method to facilitate identification of synthetic polymers using a fluorescent dye.Nile Red (NR), a fluorescent dye, which was well known to dye neutral lipid in cell and tissue sample is used in this study. It is strongly fluorescent only in the presence of a hydrophobic environment. Applicability of NR for identification and quantification of microplastics was tested and staining condition was optimized in this study. Micro-sized polyethylene (PE), polypropylene (PP) and expanded polystyrene (EPS) were used as model plastics. Stained polymer particles were recognized better in green fluorescence (Ex. 534-558 and Em. 515-565) than red (Ex. 534-558 and Em. >590). The 50mg/L solution showed higher fluorescence than 5 mg/L solution. Fluorescence intensity was in the order of EPS > PP > PE. The developed NR staining method was successfully applied to identify and quantify the fragmented polymer particles in a laboratory accelerated mechanical abrasion study. Other polyme
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25931
Bibliographic Citation
PICES 2014 Annual Meeting, pp.101, 2014
Publisher
North Pacific Marine Science Organization
Type
Conference
Language
English
Publisher
North Pacific Marine Science Organization
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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