Novel substrate specificity of a thermostable β-glucosidase from the hyperthermophilic archaeon, Thermococcus pacificus P-4 SCOPUS KCI
DC Field | Value | Language |
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dc.contributor.author | Kim, Y.J. | - |
dc.contributor.author | Lee, J.E. | - |
dc.contributor.author | Lee, H.S. | - |
dc.contributor.author | Kwon, K.K. | - |
dc.contributor.author | Kang, S.G. | - |
dc.contributor.author | Lee, J.-H. | - |
dc.date.accessioned | 2020-04-20T03:55:19Z | - |
dc.date.available | 2020-04-20T03:55:19Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0440-2413 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2588 | - |
dc.description.abstract | Based on the genomic analysis of Thermococcus pacificus P-4, we identified a putative GH1 β-glucosidase-encoding gene (Tpa-glu). The gene revealed a 1,464 bp encoding 487 amino acid residues, and the deduced amino acid residues exhibited 77% identity with Pyrococcus furiosus β-glucosidase (accession no. NP_577802). The gene was cloned and expressed in Escherichia coli system. The recombinant protein was purified by metal affinity chromatography and characterized. Tpa-Glu showed optimum activity at pH 7.5 and 75°C, and thermostability with a half life of 6 h at 90°C. Tpa-Glu exhibited hydrolyzing activity against various pNP-glycopyranosides, with kcat/Km values in the order of pNP-β-glucopyranoside, pNP-β-galactopyranoside, pNP-β-mannopyranoside, and pNP-β-xylopyranoside. In addition, the enzyme exhibited exo-hydrolyzing activity toward β-1,3-linked polysaccharide (laminarin) and β-1,3-and β-1,4-linked oligosaccharides. This is the first description of an enzyme from hyperthermophilic archaea that displays exo-hydrolyzing activity toward β-1,3-linked polysaccharides and could be applied in combination with β-1,3-endoglucanase for saccharification of laminarin. © 2015, The Microbiological Society of Korea. | - |
dc.description.uri | 3 | - |
dc.language | Korean | - |
dc.publisher | Microbiological Society of Korea | - |
dc.subject | Archaea | - |
dc.subject | Escherichia coli | - |
dc.subject | Pyrococcus furiosus | - |
dc.subject | Thermococcus pacificus | - |
dc.title | Novel substrate specificity of a thermostable β-glucosidase from the hyperthermophilic archaeon, Thermococcus pacificus P-4 | - |
dc.type | Article | - |
dc.citation.endPage | 74 | - |
dc.citation.startPage | 68 | - |
dc.citation.title | Korean Journal of Microbiology | - |
dc.citation.volume | 51 | - |
dc.citation.number | 1 | - |
dc.contributor.alternativeName | 김윤재 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 권개경 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | Korean Journal of Microbiology, v.51, no.1, pp.68 - 74 | - |
dc.identifier.doi | 10.7845/kjm.2015.5003 | - |
dc.identifier.scopusid | 2-s2.0-84938056321 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001978954 | - |
dc.description.journalClass | 3 | - |
dc.subject.keywordPlus | Archaea | - |
dc.subject.keywordPlus | Escherichia coli | - |
dc.subject.keywordPlus | Pyrococcus furiosus | - |
dc.subject.keywordPlus | Thermococcus pacificus | - |
dc.subject.keywordAuthor | Exo-hydrolyzing activity | - |
dc.subject.keywordAuthor | Laminarinase activity | - |
dc.subject.keywordAuthor | Thermococcus pacificus p-4 | - |
dc.subject.keywordAuthor | β-1,3-linked polysaccharide | - |
dc.subject.keywordAuthor | β-glucosidase | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |