The toxic effects of crude oil on the fish early life stage

Abstract

In the study, to clarify the comparative developmental effects of spilled oil between spotted bass (Lateolabrax maculates) and olive flounder (Paralichthys olivaceus) embryos (48 hr post-fertilization) were exposed to oiled gravel effluent of Iranian Heavy crude oil (IHCO) oil for 48 hrs. At 24 hrs after exposure, several complex effects, including the pericardium edema spinal curvature and developmental delay and tail mutant were observed in both of embryos after the IHCO exposure. CYP1A immunofluorescence activity was high significantly in the endothelium of head, epidermis and cardium and a significant induction of CYP1A mRNA was observed in both of embryos after the oil exposure. The extent of tail abnormalities and edema in olive flounder were stronger than those of spotted bass in oil exposed groups. But the levels of CYP1A expression were lower than those of spotted bass. Our results confirm that the HSOS caused significant damage to resident fish embryos and different toxic sensitivity were investigated between spotted bass (Lateolabrax maculates) and olive flounder (Paralichthys olivaceus) embryos in morphological and biochemical levels.of Iranian Heavy crude oil (IHCO) oil for 48 hrs. At 24 hrs after exposure, several complex effects, including the pericardium edema spinal curvature and developmental delay and tail mutant were observed in both of embryos after the IHCO exposure. CYP1A immunofluorescence activity was high significantly in the endothelium of head, epidermis and cardium and a significant induction of CYP1A mRNA was observed in both of embryos after the oil exposure. The extent of tail abnormalities and edema in olive flounder were stronger than those of spotted bass in oil exposed groups. But the levels of CYP1A expression were lower than those of spotted bass. Our results confirm that the HSOS caused significant damage to resident fish embryos and different toxic sensitivity were investigated between spotted bass (Lateolabrax maculates) and olive flounder (Paralichthys olivaceus) embryos in morphological and biochemical levels.