CO2 mineralization by the recombinant strains expressing thermostable carbonic anhydrases

Title
CO2 mineralization by the recombinant strains expressing thermostable carbonic anhydrases
Author(s)
정해창; 이성혁; 김민식; 이정현; 이현숙; 강성균
KIOST Author(s)
Jung, Hae Chang(정해창)Lee, Seong Hyuk(이성혁)Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Alternative Author(s)
정해창; 이성혁; 김민식; 이정현; 이현숙; 강성균
Publication Year
2015-04-15
Abstract
hyperthermophilic archaeon Thermococcus onnurineus NA1 emits carbon dioxide when producing H2 by oxidation of carbon monoxide or formate. As a strategy for reducing CO2 emission, we attempted to capture CO2 by precipitating to calcium carbonate minerals with the aid of carbonic anhydrase (CA). CA, one of the fastest known enzymes, is involved in the reversible hydration of CO2 to bicarbonate ions and protons and subsequently promotes the formation of CaCO3 in the presence of calcium cations. In this study, we selected thermostable CAs from thermophilic microbes such as Sulfolobus solfataricus and Metallosphera sedula, and a mesophilic CA from Acetobacterium woodii to test CA-mediated CO2 mineralization. Codon-optimized heterologous CA genes were introduced into T. onnurineus NA1 by double-crossover homologous recombination and the genes were overexpressed under the control of a strong promoter. At high temperatures, CA activity was determined by the electrometric method of Wilbur and Anderson, and CO2 mineralization was also tested by turbidity assay using UV/vis spectrophotometer. As a result, the recombinant CAs successfully accelerated CO2 mineralization to CaCO3.nate minerals with the aid of carbonic anhydrase (CA). CA, one of the fastest known enzymes, is involved in the reversible hydration of CO2 to bicarbonate ions and protons and subsequently promotes the formation of CaCO3 in the presence of calcium cations. In this study, we selected thermostable CAs from thermophilic microbes such as Sulfolobus solfataricus and Metallosphera sedula, and a mesophilic CA from Acetobacterium woodii to test CA-mediated CO2 mineralization. Codon-optimized heterologous CA genes were introduced into T. onnurineus NA1 by double-crossover homologous recombination and the genes were overexpressed under the control of a strong promoter. At high temperatures, CA activity was determined by the electrometric method of Wilbur and Anderson, and CO2 mineralization was also tested by turbidity assay using UV/vis spectrophotometer. As a result, the recombinant CAs successfully accelerated CO2 mineralization to CaCO3.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25648
Bibliographic Citation
미생물학회지/ 한국미생물학회, pp.189, 2015
Publisher
한국미생물학회/ 한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회/ 한국미생물학회
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse