Detection of Pathogenic Bacterial in Various Coral, Seaweed and Sponge Species

Title
Detection of Pathogenic Bacterial in Various Coral, Seaweed and Sponge Species
Author(s)
이다영; 이애경; 조예진; 염승식; 우선옥
KIOST Author(s)
Lee, Nayoung(이나영)Lee, Na Yun(이나윤)Jo, Yejin(조예진)Yum, Seungshic(염승식)
Alternative Author(s)
이나영; 이나윤; 조예진; 염승식; 우선옥
Publication Year
2015-05-21
Abstract
A few microorganisms have been identified as pathogenic agents responsible for various outbreaks of marine organism disease. But most of diseases have been implicated within a subset of marine organism in our knowledge of the host range and geographic extent of a given pathogen. In this study, we purposed the rapid and inexpensive detection of pathogens in various coral, seaweed and sponge species using PCR-based assays. Total 5 pathogen-specific vibrio bacteria primer sets and 2 pathogen-specific 16s rDNA primer sets were designed for detecting various coral, seaweed and sponge pathogenic disease. The pathogenic bacteria detected in coral were Aurantimonas coralicida, Serratia marcescens, Vibrio shilonii, Vibrio coralliilyticus, Vibrio carchariae, and vibrio bacteria were detected in seaweed and sponge. The assay was applied to coral samples from the Jeju Island, Japan and Taiwan, and to seaweed samples from Dokdo, Jeju, Yeosu and Uleongdo, and to sponge samples from Jeju in korea. After the identification of infected samples, the PCR-based assay is to be evaluated for detection of presence of pathogenic bacteria in various coral, seaweed and sponge.d geographic extent of a given pathogen. In this study, we purposed the rapid and inexpensive detection of pathogens in various coral, seaweed and sponge species using PCR-based assays. Total 5 pathogen-specific vibrio bacteria primer sets and 2 pathogen-specific 16s rDNA primer sets were designed for detecting various coral, seaweed and sponge pathogenic disease. The pathogenic bacteria detected in coral were Aurantimonas coralicida, Serratia marcescens, Vibrio shilonii, Vibrio coralliilyticus, Vibrio carchariae, and vibrio bacteria were detected in seaweed and sponge. The assay was applied to coral samples from the Jeju Island, Japan and Taiwan, and to seaweed samples from Dokdo, Jeju, Yeosu and Uleongdo, and to sponge samples from Jeju in korea. After the identification of infected samples, the PCR-based assay is to be evaluated for detection of presence of pathogenic bacteria in various coral, seaweed and sponge.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25481
Bibliographic Citation
한국해양학회2015춘계학회, pp.128, 2015
Publisher
(사)한국해양학회
Type
Conference
Language
English
Publisher
(사)한국해양학회
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