ENHANCEMENT OF THE PRODUCTION OF EICOSAPENTAENOIC ACID (EPA) AND FATTY ACID FROM MARINE GREEN MICROALGAE CULTURED IN F/2 MEDIA WITH THE DIFFERENT CONCENTRATIONS

Abstract

Numerous researches on essential fatty acids (EFAs) with related topics have been focused on human health improvement in worldwide. Eicosapentaenoic acid (EPA, C20:5n3) has been known to use as a functional and pharmaceutical supplementary food and to modulate of cholesterol-lowering that contribute to prevent angiostenosis [1]. Marine green microalgae contain large quantities of EPA [2], these microalgae are then considered as an industrial source in many countries. This study aimed to enhance the production of EPA and EFAs in two marine green microalgae (Tetraselmis sp. Ganghwa KCTC 12236BP and Dunaliella tertiolecta UTEX LB999) cultured in modified Guillards f/2 media (f/2, f and f*1.5 media) with the different media concentrations for 14-days in the laboratory. Furthermore, biomass and fatty acid methyl esters (FAMEs) were measured and analyzed using AOAC method [3] and GC/MS, respectively. Final biomass of Tetraselmis sp. (0.87±0.07 g/L) was higher than that of D. tertiolecta (0.63±0.03 g/L) cultured in f*1.5 medium. In this medium, Tetraselmis sp. can be grown fast during initial incubation period (2&#8211 6 days) while the cells contain enhanced concentration of EPA (2.5% to 5.2%) but the concentrations of palmitic acid (PA) and oleic acid (OA) are decreased (93.4% to 88.3%). EPA from D. tertiolecta was lower than that of Tetraselmis sp. while EFA (linoleic acid, etc.) are increased (89.0% to 93.0%). f*1.5food and to modulate of cholesterol-lowering that contribute to prevent angiostenosis [1]. Marine green microalgae contain large quantities of EPA [2], these microalgae are then considered as an industrial source in many countries. This study aimed to enhance the production of EPA and EFAs in two marine green microalgae (Tetraselmis sp. Ganghwa KCTC 12236BP and Dunaliella tertiolecta UTEX LB999) cultured in modified Guillards f/2 media (f/2, f and f*1.5 media) with the different media concentrations for 14-days in the laboratory. Furthermore, biomass and fatty acid methyl esters (FAMEs) were measured and analyzed using AOAC method [3] and GC/MS, respectively. Final biomass of Tetraselmis sp. (0.87±0.07 g/L) was higher than that of D. tertiolecta (0.63±0.03 g/L) cultured in f*1.5 medium. In this medium, Tetraselmis sp. can be grown fast during initial incubation period (2&#8211 6 days) while the cells contain enhanced concentration of EPA (2.5% to 5.2%) but the concentrations of palmitic acid (PA) and oleic acid (OA) are decreased (93.4% to 88.3%). EPA from D. tertiolecta was lower than that of Tetraselmis sp. while EFA (linoleic acid, etc.) are increased (89.0% to 93.0%). f*1.5