Anti-inflammatory effects of chromene derivatives isolated from marine algae via suppressing NF-κB and MAPK pathways
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 허수진 | - |
dc.contributor.author | 예보람 | - |
dc.contributor.author | 김민선 | - |
dc.contributor.author | 강도형 | - |
dc.contributor.author | 윤원종 | - |
dc.contributor.author | 정원교 | - |
dc.date.accessioned | 2020-07-16T00:33:53Z | - |
dc.date.available | 2020-07-16T00:33:53Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2015-06-15 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/25388 | - |
dc.description.abstract | In this study, the anti-inflammatory effect of chromene derivatives, which was isolated from Sargassum siliquastrum were evaluated by examining their inhibitory effects on pro-inflammatory mediators in LPS-stimulated murine macrophage RAW 264.7 cells. The chromene derivatives were isolated from activity-guided fraction using inhibition of NO production and identified as sargachromanol D, E, G and I on the basis of a comparison of NMR spectroscopic data. Among them, sargachromanol G (SG) was selected for further experiments due to its profound inhibitory effect in all inflammatory mediators. SG dose-dependently inhibited the production of inflammatory markers (NO, iNOS, PGE2, and COX-2) and pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of SG, we studied LPS induced NF-κB activation and MAPKs phosphorylation. SG inhibited the phosphorylation IκB-α and NF-κB (p65 and p50) and MAPK (ERK1/2, JNK, and p38) in a dose dependent manner. These results suggest that the anti-inflammatory activity of SG results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-κB activation and MAPK phosphorylation.64.7 cells. The chromene derivatives were isolated from activity-guided fraction using inhibition of NO production and identified as sargachromanol D, E, G and I on the basis of a comparison of NMR spectroscopic data. Among them, sargachromanol G (SG) was selected for further experiments due to its profound inhibitory effect in all inflammatory mediators. SG dose-dependently inhibited the production of inflammatory markers (NO, iNOS, PGE2, and COX-2) and pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of SG, we studied LPS induced NF-κB activation and MAPKs phosphorylation. SG inhibited the phosphorylation IκB-α and NF-κB (p65 and p50) and MAPK (ERK1/2, JNK, and p38) in a dose dependent manner. These results suggest that the anti-inflammatory activity of SG results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-κB activation and MAPK phosphorylation. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | (사)한국해양생명과학회 | - |
dc.relation.isPartOf | (사)한국해양생명과학회 | - |
dc.title | Anti-inflammatory effects of chromene derivatives isolated from marine algae via suppressing NF-κB and MAPK pathways | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 160 | - |
dc.citation.startPage | 160 | - |
dc.citation.title | (사)한국해양생명과학회 | - |
dc.contributor.alternativeName | 허수진 | - |
dc.contributor.alternativeName | 예보람 | - |
dc.contributor.alternativeName | 김민선 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | (사)한국해양생명과학회, pp.160 | - |
dc.description.journalClass | 2 | - |