그람 양성균의 Apo-form와 Holo-form의 구조적 분석
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 양윤모 | - |
dc.contributor.author | 이상민 | - |
dc.contributor.author | 지창준 | - |
dc.contributor.author | 이진원 | - |
dc.contributor.author | 차선신 | - |
dc.date.accessioned | 2020-07-15T23:53:49Z | - |
dc.date.available | 2020-07-15T23:53:49Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2015-09-17 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/25301 | - |
dc.description.abstract | We report the X-ray crystal structures of Gram-positive Fur proteins in both apo-form (Bacillus subtilis Fur) and Mn holo-form (Staphylococcus aureus Fur) solved by SAD (single anomalous diffraction) method. X-ray fluorescence scanning and diffraction data analysis indicate that the Mn holo-form contains 3 metals (Zn at Site 1, Mn at Site2 and Mn at Site3) per monomer and adopts a canonical closed V-shape dimeric conformation as reported previously for other DNA-binding competent Fur family proteins. Unexpectedly, the apo-form, containing only the structural Zn at Site 1, also adopts a closed V-shape dimeric conformation, but with slightly distorted orientations of DNA-binding domains. Furthermore, we solved the structure of the Zn holo-form (Zn at Site 1, Zn at Site2 and Zn at Site3) of S. aureus Fur. Despite the difference in metal coordination at Site 2 and 3, the overall structure of Zn holo-form is identical to that of Mn-holo form suggesting that Zn can also be used as a corepressor for Fur.diffraction data analysis indicate that the Mn holo-form contains 3 metals (Zn at Site 1, Mn at Site2 and Mn at Site3) per monomer and adopts a canonical closed V-shape dimeric conformation as reported previously for other DNA-binding competent Fur family proteins. Unexpectedly, the apo-form, containing only the structural Zn at Site 1, also adopts a closed V-shape dimeric conformation, but with slightly distorted orientations of DNA-binding domains. Furthermore, we solved the structure of the Zn holo-form (Zn at Site 1, Zn at Site2 and Zn at Site3) of S. aureus Fur. Despite the difference in metal coordination at Site 2 and 3, the overall structure of Zn holo-form is identical to that of Mn-holo form suggesting that Zn can also be used as a corepressor for Fur. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | KIAS | - |
dc.relation.isPartOf | The 15th KIAS Conference on Protein Structure and Function | - |
dc.title | 그람 양성균의 Apo-form와 Holo-form의 구조적 분석 | - |
dc.title.alternative | Structural Analysis of Apo-form and Holo-form of Gram-Positive Fur | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 42 | - |
dc.citation.startPage | 42 | - |
dc.citation.title | The 15th KIAS Conference on Protein Structure and Function | - |
dc.contributor.alternativeName | 양윤모 | - |
dc.contributor.alternativeName | 차선신 | - |
dc.identifier.bibliographicCitation | The 15th KIAS Conference on Protein Structure and Function, pp.42 | - |
dc.description.journalClass | 2 | - |