Enhancement of lipid content with FAMEs composition for biodiesel production from marine microalga (Tetraselmis sp.) in different culture regimes

Abstract

Recently, microalgae are mostly highlighted in the field of fuel production as third-generation of biofuels [1]. For harvesting large amount of FAMEs from microalgae, we should be assessed how the culture conditions are very effective to increase its growth with net volume of conversable fatty acids because microalgae are directly affected its own lipid synthesis by different culture conditions. The aim of this study is to enhance the lipid content with FAMEs composition of Tetraselmis sp. Ganghwa KCTC 12236BP under different regimes of f/2 medium for a month cultures in the laboratory and 20 ton-scaled outdoor culture system, and then the FAMEs and lipid contents were analyzed using GC/MS and AOAC method, respectively [2]. In the Lab-culture experiments, FAMEs of Tetraselmis sp. was estimated 93.4±0.2%, 92.4±0.5% and 88.3±0.6% under the different regimes of f/2 medium, f medium, f*1.5 medium, respectively. Especially, the average biomass of the strain in f medium (two-fold concentration of f/2 medium) was peaked up to 1.6±0.1 g/L and 18.6±0.1 g/m2/d in the laboratory and outdoor culture condition, respectively. Daily lipid productivity (PLV) of the f medium from outdoor culture (0.34±0.08 g/L/d) was slightly higher than that of f*1.5 medium (0.32±0.02 g/L/d) and then the FAMEs composition of the strain was stable at the range of 87.9-89.6% for a month cultures period. Specifically, 20 ton-scaled outdoor cultucrease its growth with net volume of conversable fatty acids because microalgae are directly affected its own lipid synthesis by different culture conditions. The aim of this study is to enhance the lipid content with FAMEs composition of Tetraselmis sp. Ganghwa KCTC 12236BP under different regimes of f/2 medium for a month cultures in the laboratory and 20 ton-scaled outdoor culture system, and then the FAMEs and lipid contents were analyzed using GC/MS and AOAC method, respectively [2]. In the Lab-culture experiments, FAMEs of Tetraselmis sp. was estimated 93.4±0.2%, 92.4±0.5% and 88.3±0.6% under the different regimes of f/2 medium, f medium, f*1.5 medium, respectively. Especially, the average biomass of the strain in f medium (two-fold concentration of f/2 medium) was peaked up to 1.6±0.1 g/L and 18.6±0.1 g/m2/d in the laboratory and outdoor culture condition, respectively. Daily lipid productivity (PLV) of the f medium from outdoor culture (0.34±0.08 g/L/d) was slightly higher than that of f*1.5 medium (0.32±0.02 g/L/d) and then the FAMEs composition of the strain was stable at the range of 87.9-89.6% for a month cultures period. Specifically, 20 ton-scaled outdoor cultu