Metabolic changes in medaka fish (Oryzias latipes) in response to acute toxicity of perfluorooctane sulfonate by heterologous microarray hybridization

Abstract

Heterologous microarray hybridization was carried out to identify the differentially expressed genes in the liver tissue of medaka fish, Oryzias latipes, after exposed to perfluorooctane sulfonate (PFOS) using an oligo-microarray (EnviHaz Fish Array ver. 01). The fish groups were exposed to 90 &#61549 g/L PFOS for 12, 24, 48, or 72 h to determine the metabolic and physiological changes with exposure time. The transcriptomic changes were highly dynamic in the PFOS-exposed fish in that among the 142 differentially expressed genes identified in all four exposed fish groups, 103 genes (72.5.1%) appeared after a specific exposure time. The differentially expressed genes were used to predict the changes that occurred in the metabolic pathways and processes in response to PFOS exposure. Many physiological and metabolic changes were detected in the early phase of exposure. The results extend our understanding of the biological responses to environmental chemicals at the molecular level and will allow the toxic effects of environmental chemicals, especially endocrine-disrupting chemicals, to be evaluated.ish Array ver. 01). The fish groups were exposed to 90 &#61549 g/L PFOS for 12, 24, 48, or 72 h to determine the metabolic and physiological changes with exposure time. The transcriptomic changes were highly dynamic in the PFOS-exposed fish in that among the 142 differentially expressed genes identified in all four exposed fish groups, 103 genes (72.5.1%) appeared after a specific exposure time. The differentially expressed genes were used to predict the changes that occurred in the metabolic pathways and processes in response to PFOS exposure. Many physiological and metabolic changes were detected in the early phase of exposure. The results extend our understanding of the biological responses to environmental chemicals at the molecular level and will allow the toxic effects of environmental chemicals, especially endocrine-disrupting chemicals, to be evaluated.