Construction of target-specific virus-like particles for the delivery of algicidal compounds to harmful algae SCIE SCOPUS

Cited 4 time in WEB OF SCIENCE Cited 4 time in Scopus
Title
Construction of target-specific virus-like particles for the delivery of algicidal compounds to harmful algae
Author(s)
Kang, Beom Sik; Eom, Chi-Yong; Kim, Wonduck; Kim, Pyoung Il; Ju, Sun Yi; Ryu, Jaewon; Han, Gui Hwan; Oh, Jeong-Il; Cho, Hoon; Baek, Seung Ho; Kim, Gueeda; Kim, Minju; Hyun, Jaekyung; Jin, EonSeon; Kim, Si Wouk
KIOST Author(s)
Baek, Seung Ho(백승호)
Publication Year
2015-04
Abstract
Harmful algal blooms (HABs) can lead to substantial socio-economic losses and extensive damage to aquatic ecosystems, drinking water sources and human health. Common algicidal techniques, including ozonation, ultrasonic treatment and dispersion of algae-killing chemicals, are unsatisfactory both economically and ecologically. This study therefore presents a novel alternative strategy for the efficient control of deleterious algae via the use of host-specific virus-like particles (VLPs) combined with chemically synthesized algicidal compounds. The capsid protein of HcRNAV34, a single-stranded RNA virus that infects the toxic dinoflagellate, Heterocapsa circularisquama, was expressed in and purified from Escherichia coli and then self-assembled into VLPsin vitro. Next, the algicidal compound, thiazolidinedione 49 (TD49), was encapsidated into HcRNAV34 VLPs for specific delivery to H. circularisquama. Consequently, HcRNAV34 VLPs demonstrated the same host selectivity as naturally occurring HcRNAV34 virions, while TD49-encapsidated VLPs showed a more potent target-specific algicidal effect than TD49 alone. These results indicate that target-specific VLPs for the delivery of cytotoxic compounds to nuisance algae might provide a safe, environmentally friendly approach for the management of HABs in aquatic ecosystems.
ISSN
1462-2912
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2512
DOI
10.1111/1462-2920.12650
Bibliographic Citation
ENVIRONMENTAL MICROBIOLOGY, v.17, no.4, pp.1463 - 1474, 2015
Publisher
WILEY
Subject
RNA VIRUS; THIAZOLIDINEDIONE DERIVATIVES; HETEROCAPSA-CIRCULARISQUAMA; CAPSID PROTEIN; RED TIDE; FLOCCULATION; HCRNAV; BLOOMS; CLAY; TD49
Type
Article
Language
English
Document Type
Article
Publisher
WILEY
Related Researcher
Research Interests

Marine Ecology,Marine Environment,HABs,해양생태,해양환경,적조

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