Annual biomass and lipid productivities of Tetraselmis sp. from onshore culture system

Abstract

Biodiesel production from microalgae has received much attention since the lipid can be converted to biodiesel reason why the lipid is mainly composed by saturated and unsaturated fatty acids. Among the many species in microalgae, saline Tetraselmis sp. is predominantly having high amount of total lipid. The aim of this study is to maintain the daily biomass production of the strain with enhancing lipid concentration using modified f/2 media (f/2, f and f*1.5 media) under the different culture volume regimes (5 L Flask, 200 L plastic tank and 20 ton raceway pond) for long-term culture experiments. Furthermore, we analyzed the total lipid content weekly-based and delivertized fatty acid methyl esters (FAMEs) using AOAC method and GC-MASS, respectively. Daily environmental conditions (solar radiation, water temp., salinity and pH etc.) were recorded during the culture times.In the Lab-culture experiments, FAMEs of Tetraselmis sp. was estimated 93.4±0.2%, 92.4±0.5% and 88.3±0.6% under the different regimes of f/2 medium, f medium and f*1.5 medium, respectively. Especially, the average biomass of the strain in f medium (two-fold concentration of f/2 medium) was peaked up to 1.6±0.1 g/L and 18.6±0.1 g/m2/d in the laboratory and outdoor culture condition, respectively. Daily lipid productivity (PLV) of the f medium from outdoor culture (0.34±0.08 g/L/d) was slightly higher than that of f*1.5 medium (0.32±0.02 g/L/traselmis sp. is predominantly having high amount of total lipid. The aim of this study is to maintain the daily biomass production of the strain with enhancing lipid concentration using modified f/2 media (f/2, f and f*1.5 media) under the different culture volume regimes (5 L Flask, 200 L plastic tank and 20 ton raceway pond) for long-term culture experiments. Furthermore, we analyzed the total lipid content weekly-based and delivertized fatty acid methyl esters (FAMEs) using AOAC method and GC-MASS, respectively. Daily environmental conditions (solar radiation, water temp., salinity and pH etc.) were recorded during the culture times.In the Lab-culture experiments, FAMEs of Tetraselmis sp. was estimated 93.4±0.2%, 92.4±0.5% and 88.3±0.6% under the different regimes of f/2 medium, f medium and f*1.5 medium, respectively. Especially, the average biomass of the strain in f medium (two-fold concentration of f/2 medium) was peaked up to 1.6±0.1 g/L and 18.6±0.1 g/m2/d in the laboratory and outdoor culture condition, respectively. Daily lipid productivity (PLV) of the f medium from outdoor culture (0.34±0.08 g/L/d) was slightly higher than that of f*1.5 medium (0.32±0.02 g/L/