Studies on the physiological function and biochemical properties of the frhAGB-encoding hydrogenase from a non-methanogenic hyperthermophilic archaeon

DC Field Value Language
dc.contributor.author 이현숙 -
dc.contributor.author 전정호 -
dc.contributor.author 임재규 -
dc.contributor.author 이정현 -
dc.contributor.author 강성균 -
dc.date.accessioned 2020-07-15T22:33:19Z -
dc.date.available 2020-07-15T22:33:19Z -
dc.date.created 2020-02-11 -
dc.date.issued 2015-12-16 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/24981 -
dc.description.abstract The F420-reducing hydrogenase has been known as a key enzyme in methanogenesis. Its homologs identified in non-methanogenic hyperthermophilic archaea were distinguished from those of most methanogens with respect to the phylogenetic analysis of the α and β subunits, organization of frhAGB genes, and conservation of F420-coordinating residues in the β subunits. The trimeric enzyme complex was purified from T. onnurineus NA1 and exhibited catalytic activity toward the electron acceptors such as viologens and flavins but not the deazaflavin coenzyme F420. The phenotypic changes associated with deletion and overexpression of frhAGB genes were investigated in T. onnurineus NA1. The deletion and overexpression of those genes enhanced growth on the CO- and formate-containing media, respectively. Transcriptional and translational levels of several hydrogenase genes were affected by frhAGB deletion, leading to the change in H2 productivity. The correlation between frhAGB-encoding hydrogenase and other hydrogenases will be discussed.s of the α and β subunits, organization of frhAGB genes, and conservation of F420-coordinating residues in the β subunits. The trimeric enzyme complex was purified from T. onnurineus NA1 and exhibited catalytic activity toward the electron acceptors such as viologens and flavins but not the deazaflavin coenzyme F420. The phenotypic changes associated with deletion and overexpression of frhAGB genes were investigated in T. onnurineus NA1. The deletion and overexpression of those genes enhanced growth on the CO- and formate-containing media, respectively. Transcriptional and translational levels of several hydrogenase genes were affected by frhAGB deletion, leading to the change in H2 productivity. The correlation between frhAGB-encoding hydrogenase and other hydrogenases will be discussed. -
dc.description.uri 1 -
dc.language English -
dc.publisher American Chemical Society -
dc.relation.isPartOf The 2015 International Chemical Congress of Pacific Basin Societies (PAC CHEM™) -
dc.title Studies on the physiological function and biochemical properties of the frhAGB-encoding hydrogenase from a non-methanogenic hyperthermophilic archaeon -
dc.type Conference -
dc.citation.conferencePlace US -
dc.citation.endPage 218 -
dc.citation.startPage 218 -
dc.citation.title The 2015 International Chemical Congress of Pacific Basin Societies (PAC CHEM™) -
dc.contributor.alternativeName 이현숙 -
dc.contributor.alternativeName 임재규 -
dc.contributor.alternativeName 이정현 -
dc.contributor.alternativeName 강성균 -
dc.identifier.bibliographicCitation The 2015 International Chemical Congress of Pacific Basin Societies (PAC CHEM™), pp.218 -
dc.description.journalClass 1 -
Appears in Collections:
Marine Resources & Environment Research Division > Marine Biotechnology &Bioresource Research Department > 2. Conference Papers
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