Dynamic Changes of Photosynthetic Picoeukaryotes Composition in the Northwestern Pacific Ocean Revealed by High-Throughput Tag Sequencing of Plastid 16S rRNA Gene

Title
Dynamic Changes of Photosynthetic Picoeukaryotes Composition in the Northwestern Pacific Ocean Revealed by High-Throughput Tag Sequencing of Plastid 16S rRNA Gene
Author(s)
노재훈; 최동한; Karen E. Selph; 이미진
KIOST Author(s)
Noh, Jae Hoon(노재훈)Choi, Dong Han(최동한)Lee, Charity Mijin(이미진)
Publication Year
2016-02-26
Abstract
Photosynthetic picoeukaryotes (PPEs) are major oceanic primary producers. However, the diversity of such communities remains poorly understood, especially in the northwestern (NW) Pacific. We investigated the abundance and diversity of PPEs, and recorded environmental variables, along a transect from the coast to the open Pacific Ocean. High-throughput tag sequencing (using the MiSeq system) revealed the diversity of plastid 16S rRNA genes. The dominant PPEs changed at the class level along the transect. Prymnesiophyceae were the only dominant PPEs in the warm pool of the NW Pacific, but Mamiellophyceae dominated in coastal waters of the East China Sea. Phylogenetically, most Prymnesiophyceae sequences could not be resolved at lower taxonomic levels because no close relatives have been cultured. Within the Mamiellophyceae, the genera Micromonas and Ostreococcus dominated in marginal coastal areas affected by open water, whereas Bathycoccus dominated in the lower euphotic depths of open oligotrophic waters. Cryptophyceae and Phaeocystis (of the Prymnesiophyceae) dominated in areas affected principally by coastal water. We also defined the biogeographical distributions of Chrysophyceae, Prasinophyceae, Bacillariophyceaea, and Pelagophyceae. These distributions were influenced by temperature, salinity, and chlorophyll a and nutrient concentrations., and recorded environmental variables, along a transect from the coast to the open Pacific Ocean. High-throughput tag sequencing (using the MiSeq system) revealed the diversity of plastid 16S rRNA genes. The dominant PPEs changed at the class level along the transect. Prymnesiophyceae were the only dominant PPEs in the warm pool of the NW Pacific, but Mamiellophyceae dominated in coastal waters of the East China Sea. Phylogenetically, most Prymnesiophyceae sequences could not be resolved at lower taxonomic levels because no close relatives have been cultured. Within the Mamiellophyceae, the genera Micromonas and Ostreococcus dominated in marginal coastal areas affected by open water, whereas Bathycoccus dominated in the lower euphotic depths of open oligotrophic waters. Cryptophyceae and Phaeocystis (of the Prymnesiophyceae) dominated in areas affected principally by coastal water. We also defined the biogeographical distributions of Chrysophyceae, Prasinophyceae, Bacillariophyceaea, and Pelagophyceae. These distributions were influenced by temperature, salinity, and chlorophyll a and nutrient concentrations.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24938
Bibliographic Citation
Ocean Science Meeting 2016, pp.1, 2016
Publisher
AGU
Type
Conference
Language
English
Publisher
AGU
Related Researcher
Research Interests

Marine Eco-Environment,Ocean legal regime/ocean policy/Ocean S&T,International Strategic Network,해양환경생태,해양법/해양정책/해양과학,전략적 국제네트워크

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