Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1 SCIE SCOPUS

Cited 6 time in WEB OF SCIENCE Cited 6 time in Scopus
Title
Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1
Author(s)
Moon, Yoon-Jung; Kwon, Joseph; Yun, Sung-Ho; Lim, Hye Li; Kim, Jonghyun; Kim, Soo Jung; Kang, Sung Gyun; Lee, Jung-Hyun; Kim, Seung Il; Chung, Young-Ho
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2015-05
Abstract
The hyperthermophilic archaeon Thermococcus onnurineus NA1 has been shown to produce H-2 when using CO, formate, or starch as a growth substrate. This strain can also utilize elemental sulfur as a terminal electron acceptor for heterotrophic growth. To gain insight into sulfur metabolism, the proteome of T. onnurineus NA1 cells grown under sulfur culture conditions was quantified and compared with those grown under H-2-evolving substrate culture conditions. Using label-free nano-UPLC-MSE-based comparative proteomic analysis, approximately 38.4% of the total identified proteome (589 proteins) was found to be significantly up-regulated (1.5-fold) under sulfur culture conditions. Many of these proteins were functionally associated with carbon fixation, Fe-S cluster biogenesis, ATP synthesis, sulfur reduction, protein glycosylation, protein translocation, and formate oxidation. Based on the abundances of the identified proteins in this and other genomic studies, the pathways associated with reductive sulfur metabolism, H-2-metabolism, and oxidative stress defense were proposed. The results also revealed markedly lower expression levels of enzymes involved in the sulfur assimilation pathway, as well as cysteine desulfurase, under sulfur culture condition. The present results provide the first global atlas of proteome changes triggered by sulfur, and may facilitate an understanding of how hyperthermophilic archaea adapt to sulfur-rich, extreme environments.
ISSN
1422-0067
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2493
DOI
10.3390/ijms16059167
Bibliographic Citation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.16, no.5, pp.9167 - 9195, 2015
Publisher
MDPI
Subject
COMPLETE GENOME SEQUENCE; DNA MICROARRAY ANALYSIS; PYROCOCCUS-FURIOSUS; ELEMENTAL SULFUR; DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE; SULFIDE DEHYDROGENASE; MEVALONATE KINASE; MEMBRANE-LIPIDS; ABC TRANSPORTER; H-2 PRODUCTION
Keywords
Thermococcus onnurineus NA1; nano-UPLC-MSE; comparative proteomics; elemental sulfur; H2S; hydrogenases; sulfur metabolism; oxidative stress defense
Type
Article
Language
English
Document Type
Article
Publisher
MDPI
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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