Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis
DC Field | Value | Language |
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dc.contributor.author | 이성혁 | - |
dc.contributor.author | 김민식 | - |
dc.contributor.author | 이재학 | - |
dc.contributor.author | 김태완 | - |
dc.contributor.author | 이성목 | - |
dc.contributor.author | 정해창 | - |
dc.contributor.author | 이정현 | - |
dc.contributor.author | 권개경 | - |
dc.contributor.author | 이현숙 | - |
dc.contributor.author | 강성균 | - |
dc.date.accessioned | 2020-07-15T21:53:22Z | - |
dc.date.available | 2020-07-15T21:53:22Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2016-04-21 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/24881 | - |
dc.description.abstract | The hyperthermophilic archaeon Thermococcus onnurineus NA1 can grow and produce H2 on carbon monoxide (CO) and its H2 production rates have been improved through metabolic engineering. Inthis study, we applied adaptive evolution to enhance H2 productivity. After over 150 serial transfers onto CO medium, cell density, CO consumption rate and H2 production rate increased. The underlying mechanism for those physiological changes could be explained by using multi-omics approaches including genomic, transcriptomic and epigenomic analyses. A putative transcriptional regulator was newly identified to regulate the expression levels of genes related to CO oxidation. Transcriptome analysis revealed significant changes in the transcript levels of genes belonging to the categories of transcription, translation and energy metabolism. Our study presents the first genome-scale methylation pattern of hyperthermophilic archaea. Adaptive evolution led to highly enhanced H2 productivity at high CO flow rates using synthesis gas produced from coal gasification.nce H2 productivity. After over 150 serial transfers onto CO medium, cell density, CO consumption rate and H2 production rate increased. The underlying mechanism for those physiological changes could be explained by using multi-omics approaches including genomic, transcriptomic and epigenomic analyses. A putative transcriptional regulator was newly identified to regulate the expression levels of genes related to CO oxidation. Transcriptome analysis revealed significant changes in the transcript levels of genes belonging to the categories of transcription, translation and energy metabolism. Our study presents the first genome-scale methylation pattern of hyperthermophilic archaea. Adaptive evolution led to highly enhanced H2 productivity at high CO flow rates using synthesis gas produced from coal gasification. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국미생물학회 | - |
dc.relation.isPartOf | 한국미생물학회 국제학술대회 | - |
dc.title | Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 195 | - |
dc.citation.startPage | 195 | - |
dc.citation.title | 한국미생물학회 국제학술대회 | - |
dc.contributor.alternativeName | 이성혁 | - |
dc.contributor.alternativeName | 김태완 | - |
dc.contributor.alternativeName | 이성목 | - |
dc.contributor.alternativeName | 정해창 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.contributor.alternativeName | 권개경 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.identifier.bibliographicCitation | 한국미생물학회 국제학술대회, pp.195 | - |
dc.description.journalClass | 2 | - |