Development of Microorganisms Based Effluent Treatment System to Remove Nitrogen and Phosphorus from Land Fish Farms

Abstract

Background: Red tide blooms have made massive economic losses and marine environmentaldisturbances in coastal region of Korea. This research was focused to develop an effectivebiological Anaerobic/Anoxic/Oxic (A2O) treatment system for Land Fish-farm with highlyefficient nitrogen and phosphorus removing bacteria and sediments.Methods: High efficientbacteria were isolated from the emerging algal blooms sites at Southern coastal of Korea. A totalof 960 strains were determined by their nitrogen and phosphorus removal efficiency.Results:From screening, Bacillus aryabhattai strain KGN1 and Vibrio neocaledonicus strain KGP1 showed more than 86.0% nitrogen and 99.9% phosphorus removal efficiency in 10 h, respectively. Interestingly, when consortia of strain KGN1 and KGP1 were made they were able to remove both nitrogen and phosphorus up to 98.0% for 10 h in a batch culture. Furthermore, both strains with sediments were employed to lab-scale A2O biological treatment system and then operated for 7 months with various operating factors such as different HRT, F/M ratio, aerobic/anaerobic time, volumetric ratio. The optimal conditions for highest effluent treatment were as HRT 6 h, F/M ratio 0.27, intermittent aeration for 30min, volumetric ratio of 1:0.95:1.4 and internal recycle of 1 Q. The reactor showed T-N of 95.6%, NH3-N of 97.3%, T-P of 97.6% and PO4 3--P of 96.0% removal efficiency, respectively. The kinetic Land Fish-farm with highlyefficient nitrogen and phosphorus removing bacteria and sediments.Methods: High efficientbacteria were isolated from the emerging algal blooms sites at Southern coastal of Korea. A totalof 960 strains were determined by their nitrogen and phosphorus removal efficiency.Results:From screening, Bacillus aryabhattai strain KGN1 and Vibrio neocaledonicus strain KGP1 showed more than 86.0% nitrogen and 99.9% phosphorus removal efficiency in 10 h, respectively. Interestingly, when consortia of strain KGN1 and KGP1 were made they were able to remove both nitrogen and phosphorus up to 98.0% for 10 h in a batch culture. Furthermore, both strains with sediments were employed to lab-scale A2O biological treatment system and then operated for 7 months with various operating factors such as different HRT, F/M ratio, aerobic/anaerobic time, volumetric ratio. The optimal conditions for highest effluent treatment were as HRT 6 h, F/M ratio 0.27, intermittent aeration for 30min, volumetric ratio of 1:0.95:1.4 and internal recycle of 1 Q. The reactor showed T-N of 95.6%, NH3-N of 97.3%, T-P of 97.6% and PO4 3--P of 96.0% removal efficiency, respectively. The kinetic