ENHANCEMENT OF BIOMASS PRODUCTION AND FATTY ACID OF TETRASELMIS SP. YH USING ORGANIC AND INORGANIC ALTERNATIVES

Abstract

Tetraselmis sp. YH was a marine green microalga and known to contain higher lipid and composition of FAMEs. It was possible to use for biofuel production and other industrial applications. Shortly, Tetraselmis sp. YH was possible to develop as commercial fatty acids source and produce large amounts of FAMEs. The aim of this study is to enhance the biomass production and FAMEs analysis of cultured Tetraselmis sp. YH using organic and inorganic alternatives. Organic and inorganic alternatives were based on modified Guillards f/2 medium added with mud extract (ME) by autoclave and organic fertilizer (OF), respectively. Furthermore, each experimental group was divided by concentration A to D group (except control) 10-day cultures in the laboratory photobioreactor culture system, and then the biomass production and FAMEs compositions were analyzed using GC/MS and AOAC method, respectively.First experiment of organic alternative, A-group (f/2:OF=3:1) of biomass production (0.45±0.03 g/L) was higher than that of control-group (0.38±0.02 g/L). Especially, total FAMEs of A-group were increased to 17% and all of FAMEs compositions were 11% increased. Also, second experiment of inorganic alternative, C-group (f/2:ME=7:1, 0.49±0.05 g/L) was higher than that of control-group (0.30±0.01 g/L). Conversely, total FAMEs of C-group and all of FAMEs compositions were decreased to 28%, 23%, respectively. Third experiment of combi as commercial fatty acids source and produce large amounts of FAMEs. The aim of this study is to enhance the biomass production and FAMEs analysis of cultured Tetraselmis sp. YH using organic and inorganic alternatives. Organic and inorganic alternatives were based on modified Guillards f/2 medium added with mud extract (ME) by autoclave and organic fertilizer (OF), respectively. Furthermore, each experimental group was divided by concentration A to D group (except control) 10-day cultures in the laboratory photobioreactor culture system, and then the biomass production and FAMEs compositions were analyzed using GC/MS and AOAC method, respectively.First experiment of organic alternative, A-group (f/2:OF=3:1) of biomass production (0.45±0.03 g/L) was higher than that of control-group (0.38±0.02 g/L). Especially, total FAMEs of A-group were increased to 17% and all of FAMEs compositions were 11% increased. Also, second experiment of inorganic alternative, C-group (f/2:ME=7:1, 0.49±0.05 g/L) was higher than that of control-group (0.30±0.01 g/L). Conversely, total FAMEs of C-group and all of FAMEs compositions were decreased to 28%, 23%, respectively. Third experiment of combi