Transcriptional responses and prediction of metabolic changes in marine medaka fish (Oryzias javanicus) to acute exposure to endocrine disrupting chemicals

Abstract

The differentially expressed genes in the liver tissue of the marine medaka fish, Oryzias javanicus, were profiled using an oligo-microarray (EnviHaz-Fish Array) which contains oligonucleotide probes for 22,796 unigenes of O. javanicus, after the fish were exposed to five kinds of endocrine disrupting chemicals (EDCs), bisphenol A (BPA), 17β-estradiol (E2), nonylphenol (NP), perfluorooctanesulfonate (PFOS), and triclosan (TCS) for 12, 24, 48, or 72 h to predict the metabolic and physiological changes with exposure time. The transcriptomic changes were highly dynamic in the EDC-exposed fish groups by the exposure time. The differentially expressed genes were used to predict the changes that occurred in the metabolic pathways and processes in response to EDCs exposure.er the fish were exposed to five kinds of endocrine disrupting chemicals (EDCs), bisphenol A (BPA), 17β-estradiol (E2), nonylphenol (NP), perfluorooctanesulfonate (PFOS), and triclosan (TCS) for 12, 24, 48, or 72 h to predict the metabolic and physiological changes with exposure time. The transcriptomic changes were highly dynamic in the EDC-exposed fish groups by the exposure time. The differentially expressed genes were used to predict the changes that occurred in the metabolic pathways and processes in response to EDCs exposure.