Enhancement of Total Lipid with FAMEs Compositions of Tetraselmis sp. YH by Modified Media using Organic and Inorganic Alternatives

Abstract

The aim of this study is to enhance the total lipid with FAMEs compositions of Tetraselmis sp. YH using by modified media using organic and inorganic alternatives. The following culture experimental design was used to evaluate the effects of organic and inorganic alternatives: each experimental group was made from modified Guillards f/2 medium with mud extract (ME) by autoclave and organic fertilizer (OF). Furthermore, A- to D-group (except control) was divided by concentrations of organic and inorganic alternatives in the laboratory photobioreactor culture system (10-days culture). Then analyzed total lipid and FAMEs compositions using AOAC and GC/MS methods, respectively. In an experiment using organic alternative, biomass production (0.45±0.03 g/L) of A-group experiment (a ratio of f/2:OF=3:1) was higher than that of control-group (0.38±0.02 g/L). Especially, the total lipid of A-group was 14.1±0.2%, and FAMEs compositions were recorded to 53.61 mg/g. In an experiment using inorganic alternative, biomass production (0.49±0.05 g/L) of C-group (a ratio of f/2:ME=7:1) was higher than that of control-group (0.30±0.01 g/L) while total lipid and FAMEs contents were 11.7±0.1% and 67.38 mg/g, respectively. In an experiment using the cocktail of organic and inorganic alternatives, biomass production (0.66±0.03 g/L) of D-group (a ratio of f/2:OF:ME=8:1:7) was highest than that of control-group (0.44±0.04 g/L). Parf organic and inorganic alternatives: each experimental group was made from modified Guillards f/2 medium with mud extract (ME) by autoclave and organic fertilizer (OF). Furthermore, A- to D-group (except control) was divided by concentrations of organic and inorganic alternatives in the laboratory photobioreactor culture system (10-days culture). Then analyzed total lipid and FAMEs compositions using AOAC and GC/MS methods, respectively. In an experiment using organic alternative, biomass production (0.45±0.03 g/L) of A-group experiment (a ratio of f/2:OF=3:1) was higher than that of control-group (0.38±0.02 g/L). Especially, the total lipid of A-group was 14.1±0.2%, and FAMEs compositions were recorded to 53.61 mg/g. In an experiment using inorganic alternative, biomass production (0.49±0.05 g/L) of C-group (a ratio of f/2:ME=7:1) was higher than that of control-group (0.30±0.01 g/L) while total lipid and FAMEs contents were 11.7±0.1% and 67.38 mg/g, respectively. In an experiment using the cocktail of organic and inorganic alternatives, biomass production (0.66±0.03 g/L) of D-group (a ratio of f/2:OF:ME=8:1:7) was highest than that of control-group (0.44±0.04 g/L). Par