Enhancement of Total Lipid with FAMEs Compositions of Tetraselmis sp. YH by Modified Media using Organic and Inorganic Alternatives

Title
Enhancement of Total Lipid with FAMEs Compositions of Tetraselmis sp. YH by Modified Media using Organic and Inorganic Alternatives
Author(s)
최우석; 김태호; 이경아; 박아름이; 강도형
KIOST Author(s)
Kim, Taeho(김태호)Park, Areumi(박아름이)Kang, Do-Hyung(강도형)
Publication Year
2016-09-30
Abstract
The aim of this study is to enhance the total lipid with FAMEs compositions of Tetraselmis sp. YH using by modified media using organic and inorganic alternatives. The following culture experimental design was used to evaluate the effects of organic and inorganic alternatives: each experimental group was made from modified Guillards f/2 medium with mud extract (ME) by autoclave and organic fertilizer (OF). Furthermore, A- to D-group (except control) was divided by concentrations of organic and inorganic alternatives in the laboratory photobioreactor culture system (10-days culture). Then analyzed total lipid and FAMEs compositions using AOAC and GC/MS methods, respectively. In an experiment using organic alternative, biomass production (0.45±0.03 g/L) of A-group experiment (a ratio of f/2:OF=3:1) was higher than that of control-group (0.38±0.02 g/L). Especially, the total lipid of A-group was 14.1±0.2%, and FAMEs compositions were recorded to 53.61 mg/g. In an experiment using inorganic alternative, biomass production (0.49±0.05 g/L) of C-group (a ratio of f/2:ME=7:1) was higher than that of control-group (0.30±0.01 g/L) while total lipid and FAMEs contents were 11.7±0.1% and 67.38 mg/g, respectively. In an experiment using the cocktail of organic and inorganic alternatives, biomass production (0.66±0.03 g/L) of D-group (a ratio of f/2:OF:ME=8:1:7) was highest than that of control-group (0.44±0.04 g/L). Parf organic and inorganic alternatives: each experimental group was made from modified Guillards f/2 medium with mud extract (ME) by autoclave and organic fertilizer (OF). Furthermore, A- to D-group (except control) was divided by concentrations of organic and inorganic alternatives in the laboratory photobioreactor culture system (10-days culture). Then analyzed total lipid and FAMEs compositions using AOAC and GC/MS methods, respectively. In an experiment using organic alternative, biomass production (0.45±0.03 g/L) of A-group experiment (a ratio of f/2:OF=3:1) was higher than that of control-group (0.38±0.02 g/L). Especially, the total lipid of A-group was 14.1±0.2%, and FAMEs compositions were recorded to 53.61 mg/g. In an experiment using inorganic alternative, biomass production (0.49±0.05 g/L) of C-group (a ratio of f/2:ME=7:1) was higher than that of control-group (0.30±0.01 g/L) while total lipid and FAMEs contents were 11.7±0.1% and 67.38 mg/g, respectively. In an experiment using the cocktail of organic and inorganic alternatives, biomass production (0.66±0.03 g/L) of D-group (a ratio of f/2:OF:ME=8:1:7) was highest than that of control-group (0.44±0.04 g/L). Par
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24581
Bibliographic Citation
제 30회 한국조류학회 학술발표대회 및 국제심포지엄, pp.77, 2016
Publisher
(사)한국조류학회
Type
Conference
Language
English
Publisher
(사)한국조류학회
Related Researcher
Research Interests

Microalgae Culture and Application,Aquaculture System Engineering,미세조류 배양 및 응용,배양시스템 엔지니어링,유용해양생물 배양 및 응용

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