Enhanced hydrogen production by a laboratory-evolved archaeon in formate

Title
Enhanced hydrogen production by a laboratory-evolved archaeon in formate
Author(s)
정해창; 이성혁; 이정현; 이현숙; 강성균
KIOST Author(s)
Jung, Hae Chang(정해창)Lee, Seong Hyuk(이성혁)Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2016-10-06
Abstract
The hyperthermophilic archaeon Thermococcus onnurineus NA1 has been reported to grow on formate coupled with H2 production and ATP generation (HCOO- + H2O→HCO3- + H2, ΔG80oC = -2.6 kJ/mol). In this study, we applied adaptive evolution toimprove H2 productivity of the strain. The strain was grown in a medium containing formate and then transferred to a fresh medium every 15 hours. Through serial transfers, physiological changes were monitored and gradual increases in cell density, H2 production and formate consumption were observed. After 156 transfers, the evolved strain showed 1.7-, 1.9- and 1.9-fold higher values in cell density, H2 production and formate consumption in comparison with those of the parental strain. In order to obtain an integrative understanding of the genetic and phenotypic changes during evolution, genome sequencing was performed and 11 mutation sites, including 2 insertions, 2 deletions and 7 substitutions either at the intergenic (2 sites) or coding regions (9 sites), were observed. When we introduced each reverse mutation in the evolved strain, two revertants for TON_1561 and TON_1573 encoding F420-reducing hydrogenase β subunit and formate transporter,respectively exhibited significant decreases in the cell density and hydrogen production. On the other hand, the mutations in TON_1561 or TON_1573 were introduced to the wild-type strain and only TON_1573 A52T mutant displayed increases ofmprove H2 productivity of the strain. The strain was grown in a medium containing formate and then transferred to a fresh medium every 15 hours. Through serial transfers, physiological changes were monitored and gradual increases in cell density, H2 production and formate consumption were observed. After 156 transfers, the evolved strain showed 1.7-, 1.9- and 1.9-fold higher values in cell density, H2 production and formate consumption in comparison with those of the parental strain. In order to obtain an integrative understanding of the genetic and phenotypic changes during evolution, genome sequencing was performed and 11 mutation sites, including 2 insertions, 2 deletions and 7 substitutions either at the intergenic (2 sites) or coding regions (9 sites), were observed. When we introduced each reverse mutation in the evolved strain, two revertants for TON_1561 and TON_1573 encoding F420-reducing hydrogenase β subunit and formate transporter,respectively exhibited significant decreases in the cell density and hydrogen production. On the other hand, the mutations in TON_1561 or TON_1573 were introduced to the wild-type strain and only TON_1573 A52T mutant displayed increases of
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24573
Bibliographic Citation
2016 Asian Biohydrogen & Biogas Symposium, pp.112, 2016
Publisher
2016
Type
Conference
Language
English
Publisher
2016
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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