Extracellular Vesicles of the Hyperthermophilic Archaeon "Thermococcus onnurineus" NA1(T)
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Title
- Extracellular Vesicles of the Hyperthermophilic Archaeon "Thermococcus onnurineus" NA1(T)
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Author(s)
- Choi, Dong Hee; Kwon, Yong Min; Chiura, Hiroshi Xavier; Yang, Eun Chan; Bae, Seung Seob; Kang, Sung Gyun; Lee, Jung-Hyun; Yoon, Hwan Su; Kim, Sang-Jin
- KIOST Author(s)
- Yang, Eun Chan(양은찬); Kang, Sung Gyun(강성균); Lee, Jung Hyun(이정현)
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Alternative Author(s)
- 최동희; 권용민; 양은찬; 배승섭; 강성균; 이정현; 김상진
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Publication Year
- 2015-07
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Abstract
- Extracellular vesicles (EVs) produced by a sulfur-reducing, hyperthermophilic archaeon, "Thermococcus onnurineus" NA1(T), were purified and characterized. A maximum of four EV bands, showing buoyant densities between 1.1899 and 1.2828 g cm(-3), were observed after CsCl ultracentrifugation. The two major EV bands, B (buoyant density at 25 degrees C [rho(25)] = 1.2434 g cm(-3)) and C (rho(25) = 1.2648 g cm(-3)), were separately purified and counted using a qNano particle analyzer. These EVs, showing different buoyant densities, were identically spherical in shape, and their sizes varied from 80 to 210 nm in diameter, with 120- and 190-nm sizes predominant. The average size of DNA packaged into EVs was about 14 kb. The DNA of the EVs in band C was sequenced and assembled. Mapping of the T. onnurineus NA1(T) EV (ToEV) DNA sequences onto the reference genome of the parent archaeon revealed that most genes of T. onnurineus NA1(T) were packaged into EVs, except for an similar to 9.4-kb region from TON_0536 to TON_0544. The absence of this specific region of the genome in the EVs was confirmed from band B of the same culture and from bands B and C purified from a different batch culture. The presence of the 3'-terminal sequence and the absence of the 5'-terminal sequence of TON_0536 were repeatedly confirmed. On the basis of these results, we hypothesize that the unpackaged part of the T. onnurineus NA1(T) genome might be related to the process that delivers DNA into ToEVs and/or the mechanism generating the ToEVs themselves.
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ISSN
- 0099-2240
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2452
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DOI
- 10.1128/AEM.00428-15
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Bibliographic Citation
- APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.81, no.14, pp.4591 - 4599, 2015
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Publisher
- AMER SOC MICROBIOLOGY
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Subject
- OUTER-MEMBRANE VESICLES; PROTEOME ANALYSIS; H-2 PRODUCTION; DNA; TRANSFORMATION; REVEALS; VIRUSES; ENUMERATION; SULFOLOBUS; MICROSCOPY
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Type
- Article
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Language
- English
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Document Type
- Article
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