The complete mitochondrial genome of the Goldeye rockfish Sebastes thompsoni (Scorpaenidae, Scorpaeniformes)

Abstract

The goldeye rockfish Sebastes thompsoni, a temperate North Pacific viviparous fish, is a commercially important species in Korea. The S. thompsoni lives in association with drifting seaweed during the early developmental period. While adult goldeye rockfish inhabits the coastal rocky bottom at about 100 m depth. To find some useful genetic information on the genus Sebastes, the complete mitochondrial genome of the S. thompsoni was determined using next-generation sequencing, including Sanger sequencing. The complete mitochondrial DNA sequence of S. thompsoni was 16,405 bp in length (GenBank Accession No. KJ834064). Its mitogenome is composed of 2 rRNAs, 22 tRNAs, and 13 PCGs, as well as the control region. The nucleotide composition in the mitogenome was 28.0% A, 26.8% T, 17.0% G, and 28.2% C, showing a slight AT bias (54.7%). The typical start codon was ATG in 12 PCGs with the exception of COX1 (GTG). The termination codon of PCGs was mainly TAR with two exceptions: ND4 (AGA) and Cytb (incomplete codon, T). The tRNA genes were 67&#8211 74 bp in length. The lengths of the 12S rRNA, 16S rRNA and control region were 946 bp, 1692 bp, and 740 bp, respectively. These gene structures, positions, and lengths were similar to those of other Sebastinae fish. Sebastes thompsoni was clearly separated from the other three genera in the phylogenetic tree of Scorpaeniformes using 2 rRNA and 13 PCGs. The most related species was Seb goldeye rockfish inhabits the coastal rocky bottom at about 100 m depth. To find some useful genetic information on the genus Sebastes, the complete mitochondrial genome of the S. thompsoni was determined using next-generation sequencing, including Sanger sequencing. The complete mitochondrial DNA sequence of S. thompsoni was 16,405 bp in length (GenBank Accession No. KJ834064). Its mitogenome is composed of 2 rRNAs, 22 tRNAs, and 13 PCGs, as well as the control region. The nucleotide composition in the mitogenome was 28.0% A, 26.8% T, 17.0% G, and 28.2% C, showing a slight AT bias (54.7%). The typical start codon was ATG in 12 PCGs with the exception of COX1 (GTG). The termination codon of PCGs was mainly TAR with two exceptions: ND4 (AGA) and Cytb (incomplete codon, T). The tRNA genes were 67&#8211 74 bp in length. The lengths of the 12S rRNA, 16S rRNA and control region were 946 bp, 1692 bp, and 740 bp, respectively. These gene structures, positions, and lengths were similar to those of other Sebastinae fish. Sebastes thompsoni was clearly separated from the other three genera in the phylogenetic tree of Scorpaeniformes using 2 rRNA and 13 PCGs. The most related species was Seb