Rapid Diagnosis of Necrosis Nervous Virus Infection using Recombinant Single-chain Variable Fragment (scFv)

DC Field Value Language
dc.contributor.author 서한열 -
dc.contributor.author 박동훈 -
dc.contributor.author 조문주 -
dc.contributor.author 조승찬 -
dc.contributor.author 김동준 -
dc.contributor.author 이용준 -
dc.contributor.author 박소윤 -
dc.contributor.author 이택견 -
dc.contributor.author 이석찬 -
dc.date.accessioned 2020-07-15T19:33:24Z -
dc.date.available 2020-07-15T19:33:24Z -
dc.date.created 2020-02-11 -
dc.date.issued 2016-11-04 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/24369 -
dc.description.abstract Many fishes have been infected with many kinds of marine pathogens and died, but marine viruses are most threatening pathogens. Marine viruses show a high lethality of ~70% but there is no therapeutic method for virus-infected fishes. Therefore, detection of marine viruses is very important issue for fish pathology. Marine virus Nervous Necrosis Virus (NNV), which is one of the most important marine viruses in Korea, infects high demanded fishes such as Paralichthys olivaceus and Scomber japonicas. NNV belongs to the family Nodaviridae, which has ssRNA (+) genome. This virus can be transmitted not only by tissues from infected fish but also by passive diffusion. Single Chain Variable Fragments (scFv) was chosen to detection and therapeutic methods in this study. Detection methods using scFvs has shown simplicity, speed, relatively high specificity and sensitivity than ordinary antibodies-using methods. To screen specific scFvs, viral coat protein as antigen is expressed by yeast surface display, which has advantages of post-translational process and efficient to use flow cytometry. scFvs expressed by phage display was used for ‘bio-panning’ process to get specific scFvs bound to antigen. After screening specific scFvs to antigen, they will be used to develop the marine virus detection kit. -
dc.description.uri 1 -
dc.language English -
dc.publisher The Korean Sciety of Virology -
dc.relation.isPartOf International Meeting of the Federation of Korean Microbiological Societies -
dc.title Rapid Diagnosis of Necrosis Nervous Virus Infection using Recombinant Single-chain Variable Fragment (scFv) -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 303 -
dc.citation.startPage 303 -
dc.citation.title International Meeting of the Federation of Korean Microbiological Societies -
dc.identifier.bibliographicCitation International Meeting of the Federation of Korean Microbiological Societies, pp.303 -
dc.description.journalClass 1 -
Appears in Collections:
South Sea Research Institute > Risk Assessment Research Center > 2. Conference Papers
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse