Chitosanase signal peptide from Bacillus subtilis its lead target protein to periplasmic space in Escherichia coli
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오철홍 | - |
dc.contributor.author | 문송 | - |
dc.contributor.author | 조은영 | - |
dc.contributor.author | 이수진 | - |
dc.contributor.author | 이영득 | - |
dc.contributor.author | 박건후 | - |
dc.contributor.author | 권영경 | - |
dc.contributor.author | Hettiarachchi Sachithra Amarin | - |
dc.contributor.author | 강도형 | - |
dc.date.accessioned | 2020-07-15T19:32:45Z | - |
dc.date.available | 2020-07-15T19:32:45Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2016-11-10 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/24345 | - |
dc.description.abstract | We isolated chitosanase secreting B. subtilis CH2 and identified the chitosanase sequence. Analyzed the sequence showed that it consisted of 813 bp, including 87 bp signal sequence. The signal sequence leads the target protein to the cell-membrane of the B. subtilis CH2 and then secret the chitosanase out of the cell. The signal peptide showed 6 amino acid deletion compared to other B. subtilis chitosnase signal peptides. The chitosanase sequence including signal peptide was cloned into pET11a vector without fusion and expressed in E. coli BL21(DE3). The expressed chitosanase in E. coli showed two distinct bands which represent the pro-chitosanase in cytoplasm and mature chitosanase in periplasm. Time frame induction and results showed that muture chitosanase was increased. Subsequently, we linked this chitosanase signal sequence in front of B. subtilis CH2 mature lichenase and xylanase sequences, and expressed it in E. coli BL21(DE3). The recombinant xylanase moved to periplasmic space. However, the recombinant lichenase did not move to periplasm.embrane of the B. subtilis CH2 and then secret the chitosanase out of the cell. The signal peptide showed 6 amino acid deletion compared to other B. subtilis chitosnase signal peptides. The chitosanase sequence including signal peptide was cloned into pET11a vector without fusion and expressed in E. coli BL21(DE3). The expressed chitosanase in E. coli showed two distinct bands which represent the pro-chitosanase in cytoplasm and mature chitosanase in periplasm. Time frame induction and results showed that muture chitosanase was increased. Subsequently, we linked this chitosanase signal sequence in front of B. subtilis CH2 mature lichenase and xylanase sequences, and expressed it in E. coli BL21(DE3). The recombinant xylanase moved to periplasmic space. However, the recombinant lichenase did not move to periplasm. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | The Genetics Society of Korea | - |
dc.relation.isPartOf | International Conference of the Genetics Society of Korea 2016 | - |
dc.title | Chitosanase signal peptide from Bacillus subtilis its lead target protein to periplasmic space in Escherichia coli | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 182 | - |
dc.citation.startPage | 182 | - |
dc.citation.title | International Conference of the Genetics Society of Korea 2016 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.contributor.alternativeName | 문송 | - |
dc.contributor.alternativeName | 조은영 | - |
dc.contributor.alternativeName | 이수진 | - |
dc.contributor.alternativeName | 이영득 | - |
dc.contributor.alternativeName | 박건후 | - |
dc.contributor.alternativeName | 권영경 | - |
dc.contributor.alternativeName | Amarin | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | International Conference of the Genetics Society of Korea 2016, pp.182 | - |
dc.description.journalClass | 1 | - |