Development of a rapid kit for harmful algal detection

Abstract

Invention of more quick, accurate, and efficient method which could have predictive and prognostic function is necessary to overcome the difficulties of conventional method for microorganisms monitoring. We have challenged to develop the immunochromatography based rapid kit for microalgae detection. As a result a rapid kit using monoclonal antibodies (mAbs) raised against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit of Alexandrium tamarense, a candidate of harmful algal bloom species in the coast of Korea. The western blot analysis showed that the mAb detect five dinoflagellates, A. tamarense, H.circularisquama, H.pygmaea, A.sanguinea and C.polykrikoides. But do not detect H.triquetra, S.costatum, C.closterium. The mAb showed no signal in one dinoflagellate and two diatom species, C.closterium, and S.costatum. The rapid kit showed a positive signal at about 1,000 cells of A. tamarense and H.triquetra, 50,000 cells of A.sanguinea and so on.munochromatography based rapid kit for microalgae detection. As a result a rapid kit using monoclonal antibodies (mAbs) raised against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit of Alexandrium tamarense, a candidate of harmful algal bloom species in the coast of Korea. The western blot analysis showed that the mAb detect five dinoflagellates, A. tamarense, H.circularisquama, H.pygmaea, A.sanguinea and C.polykrikoides. But do not detect H.triquetra, S.costatum, C.closterium. The mAb showed no signal in one dinoflagellate and two diatom species, C.closterium, and S.costatum. The rapid kit showed a positive signal at about 1,000 cells of A. tamarense and H.triquetra, 50,000 cells of A.sanguinea and so on.