초고온성 고세균 Thermococcus onnurineus에 의한 제철소 부생가스로부터 바이오수소 연속생산 실증연구

Title
초고온성 고세균 Thermococcus onnurineus에 의한 제철소 부생가스로부터 바이오수소 연속생산 실증연구
Alternative Title
Demonstration of the Bio-hydrogen Production from Steel Mill By-product Gas by the continuous culture of the Hyperthermophilic Archaeon, Thermococcus onnurineus in a Pilot Scale Bio-reactor
Author(s)
김태완; 이성목; 나정걸; 이성혁; 임재규; 김민식; 조강익; 이현숙; 권개경; 이정현; 강성균
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Lee, Hyun Sook(이현숙)Kwon, Kae Kyoung(권개경)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)
Publication Year
2016-11-26
Abstract
Thermococcus onnurineus, which had been isolated from a deep-sea hydrothermal vent area in the PACMANUS field in 2002, has been recently found to be able to grow on carbon monoxide (CO) producing hydrogen (H2) via water-gas shift (WGS) reaction. In this study, using steel mill by-product gas as a CO source, the mass production of bio-H2 was demonstrated by long-term continuous culture of T. onnurineus in a 1,000L gas-lift bio-reactor equipped with six external gas-liquid mixers. Of the steel mill by-product gases, Linz-Donawitz gas (LDG) containing ca. 55% of CO was used throughout the experiment. The continuous cultivation of T. onnurineus was carried out for 40 days varying gas supply rate of LDG or gauge pressure in a reactor at fixed dilution rate of 0.042 h-1. As a result, it was observed that both the cell growth and the volumetric H2 production rate (HPR) were increased depending on the LDG supply rate mainly due to the increase of gas-liquid mass transfer rate. Whereas, the CO conversion showed an inversely proportional relationship to the LDG supply rate probably due to the reduced residence time of CO by the increased superficial velocity of gas. The gauge pressure in a reactor was also turned out to be a key process variable in WGS activity of T. onnurineus showing the positive effect in cell growth, HPR and CO conversion. At LDG supply rate of 0.2 vvm and gauge pressure of 1.5 bar, the cell concentrationtion. In this study, using steel mill by-product gas as a CO source, the mass production of bio-H2 was demonstrated by long-term continuous culture of T. onnurineus in a 1,000L gas-lift bio-reactor equipped with six external gas-liquid mixers. Of the steel mill by-product gases, Linz-Donawitz gas (LDG) containing ca. 55% of CO was used throughout the experiment. The continuous cultivation of T. onnurineus was carried out for 40 days varying gas supply rate of LDG or gauge pressure in a reactor at fixed dilution rate of 0.042 h-1. As a result, it was observed that both the cell growth and the volumetric H2 production rate (HPR) were increased depending on the LDG supply rate mainly due to the increase of gas-liquid mass transfer rate. Whereas, the CO conversion showed an inversely proportional relationship to the LDG supply rate probably due to the reduced residence time of CO by the increased superficial velocity of gas. The gauge pressure in a reactor was also turned out to be a key process variable in WGS activity of T. onnurineus showing the positive effect in cell growth, HPR and CO conversion. At LDG supply rate of 0.2 vvm and gauge pressure of 1.5 bar, the cell concentration
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24281
Bibliographic Citation
한국해양바이오학회 정기학술대회, pp.74, 2016
Publisher
한국해양바이오학회
Type
Conference
Language
English
Publisher
한국해양바이오학회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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