Anti-inflammatory effect of Heterosigma akashiwo in lipopolysaccharide-stimulated RAW 264.7 macrophages
DC Field | Value | Language |
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dc.contributor.author | 김준성 | - |
dc.contributor.author | 최윤경 | - |
dc.contributor.author | 예보람 | - |
dc.contributor.author | 김은아 | - |
dc.contributor.author | 허수진 | - |
dc.date.accessioned | 2020-07-15T18:54:59Z | - |
dc.date.available | 2020-07-15T18:54:59Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2016-11-29 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/24276 | - |
dc.description.abstract | Red tide Heterosigma akashiwo (H. akashiwo), a microscopic alga of the Raphidophyceae, causes much damage to all marine ecosystem. Reduce damage to marine ecosystems want to use as a resource. In this study, Ethyl acetate fraction-Methanol fraction (HAEM) obtained from H. akashiwo using organic solvent fractionation and then evaluated anti-inflammatory activity in LPS-stimulated RAW 264.7 cells and zebrafish model. The result exhibited that HAME80 markedly inhibited the productions of nitric oxide (NO) and prostaglandin E2 (PGE2). Also, HAEM80 down-regulated the protein expression levels of inducible nitric oxide synthase (iNOS) and Cyclooxygenase (COX-2) as well as the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. In further study, HAME80 improved the survival rate and decreased the yolk edema in LPS-treated zebrafish embryo. Moreover, HAME80 significantly reduced the production of ROS and NO and the cell death induced by LPS- stimulated in zebrafish embryo. The peak was confirmed 1to20 by gas chromatography mass spectrometry (GC-MS). Therefore, we suggest that H. akashiwo could be used as a beneficial anti-inflammatory agent.fraction (HAEM) obtained from H. akashiwo using organic solvent fractionation and then evaluated anti-inflammatory activity in LPS-stimulated RAW 264.7 cells and zebrafish model. The result exhibited that HAME80 markedly inhibited the productions of nitric oxide (NO) and prostaglandin E2 (PGE2). Also, HAEM80 down-regulated the protein expression levels of inducible nitric oxide synthase (iNOS) and Cyclooxygenase (COX-2) as well as the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. In further study, HAME80 improved the survival rate and decreased the yolk edema in LPS-treated zebrafish embryo. Moreover, HAME80 significantly reduced the production of ROS and NO and the cell death induced by LPS- stimulated in zebrafish embryo. The peak was confirmed 1to20 by gas chromatography mass spectrometry (GC-MS). Therefore, we suggest that H. akashiwo could be used as a beneficial anti-inflammatory agent. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국해양바이오학회 | - |
dc.relation.isPartOf | 한국해양바이오학회 정기총회 및 학술발표회 | - |
dc.title | Anti-inflammatory effect of Heterosigma akashiwo in lipopolysaccharide-stimulated RAW 264.7 macrophages | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 171 | - |
dc.citation.startPage | 171 | - |
dc.citation.title | 한국해양바이오학회 정기총회 및 학술발표회 | - |
dc.contributor.alternativeName | 김준성 | - |
dc.contributor.alternativeName | 최윤경 | - |
dc.contributor.alternativeName | 예보람 | - |
dc.contributor.alternativeName | 김은아 | - |
dc.contributor.alternativeName | 허수진 | - |
dc.identifier.bibliographicCitation | 한국해양바이오학회 정기총회 및 학술발표회, pp.171 | - |
dc.description.journalClass | 2 | - |