Planktonic bivalve larvae identification and quantification in Gomso Bay, South Korea, using next-generation sequencing analysis and microscopic observations

DC Field Value Language
dc.contributor.author 정승원 -
dc.date.accessioned 2020-07-15T15:54:15Z -
dc.date.available 2020-07-15T15:54:15Z -
dc.date.created 2020-02-11 -
dc.date.issued 2017-04-19 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/24101 -
dc.description.abstract The planktonic larval stages of bivalves are notoriously difficult to identify due to their similar morphological characteristics. To identify planktonic bivalve larvae and evaluate changes in their abundance patterns, three different sites within Gomso Bay (Yellow Sea, South Korea), were twice a month sampled and analysed using a next-generation sequencing (NGS) and microscopic observations. Although these techniques identified 114 and 112 taxa, respectively, they revealed different patterns, and at least five bivalve taxa were only detected by NGS. According to NGS results, Crassostrea gigas was the most abundant bivalve larvae (37%), followed by Ruditapes philippinarum (26%) and Solen strictus (23%) Mactra chinensis and M. veneriformis accounted for less than 1% of the bivalve larvae. Crassostrea gigas and S. strictus were the most abundant in late June and rare in the other investigated periods, while R. philippinarum was more abundant in June and late September. Microscopic observations showed a similar trend to NGS in terms of bivalve larvae presence, although bivalve larvae accounted for less than 0.0001% of the plankton communities according to morphological data. Thus, morphological and molecular approaches should be combined in future studies to provide accurate sequences of correctly identified specimens to develop NGS libraries. within Gomso Bay (Yellow Sea, South Korea), were twice a month sampled and analysed using a next-generation sequencing (NGS) and microscopic observations. Although these techniques identified 114 and 112 taxa, respectively, they revealed different patterns, and at least five bivalve taxa were only detected by NGS. According to NGS results, Crassostrea gigas was the most abundant bivalve larvae (37%), followed by Ruditapes philippinarum (26%) and Solen strictus (23%) Mactra chinensis and M. veneriformis accounted for less than 1% of the bivalve larvae. Crassostrea gigas and S. strictus were the most abundant in late June and rare in the other investigated periods, while R. philippinarum was more abundant in June and late September. Microscopic observations showed a similar trend to NGS in terms of bivalve larvae presence, although bivalve larvae accounted for less than 0.0001% of the plankton communities according to morphological data. Thus, morphological and molecular approaches should be combined in future studies to provide accurate sequences of correctly identified specimens to develop NGS libraries. -
dc.description.uri 2 -
dc.language English -
dc.publisher 한국해양학회 -
dc.relation.isPartOf 한국해양학회 -
dc.title Planktonic bivalve larvae identification and quantification in Gomso Bay, South Korea, using next-generation sequencing analysis and microscopic observations -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 105 -
dc.citation.startPage 105 -
dc.citation.title 한국해양학회 -
dc.identifier.bibliographicCitation 한국해양학회, pp.105 -
dc.description.journalClass 2 -
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South Sea Research Institute > Library of Marine Samples > 2. Conference Papers
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