The study on pathogenic bacteria in coral, Screlonepthya gracillimum

Abstract

In this study, we purposed the rapid and inexpensive detection of pathogens in coral species using PCR-based assays. Total 4 pathogen-specific 16s rDNA primer sets and 1 Vibrio choleras Lux R gene primer set were designed for detecting coral pathogenic disease. The pathogenic bacteria used in this study are Aurantimonas coralicida, Serratia marcescens, Vibrio shilonii, Vibrio coralliilyticus, and Vibrio carchariae. The assay was applied to coral samples from the Jeju Island,Japan and Taiwan (Jeju Island -Sungsan, Munsum Japan - Wakayama , Kochi Taiwan - Green Island, Kenting). After the identification of infected samples, the PCR-based assay is to be evaluated for detection of presence of pathogenic bacteria in corals.l pathogenic disease. The pathogenic bacteria used in this study are Aurantimonas coralicida, Serratia marcescens, Vibrio shilonii, Vibrio coralliilyticus, and Vibrio carchariae. The assay was applied to coral samples from the Jeju Island,Japan and Taiwan (Jeju Island -Sungsan, Munsum Japan - Wakayama , Kochi Taiwan - Green Island, Kenting). After the identification of infected samples, the PCR-based assay is to be evaluated for detection of presence of pathogenic bacteria in corals.