Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate

DC Field Value Language
dc.contributor.author 정해창 -
dc.contributor.author 이성혁 -
dc.contributor.author 이성목 -
dc.contributor.author 안영준 -
dc.contributor.author 이정현 -
dc.contributor.author 이현숙 -
dc.contributor.author 강성균 -
dc.date.accessioned 2020-07-15T14:53:03Z -
dc.date.available 2020-07-15T14:53:03Z -
dc.date.created 2020-02-11 -
dc.date.issued 2017-08-24 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/23867 -
dc.description.abstract Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could growon formate and produce H2. Formate conversion to hydrogen was mediated by a formate-hydrogenlyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecularchanges. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H2 production and formate consumption increased remarkably. The 156 times transferred-strain, WTF-156T, was demonstrated to enhance H2 production using formate in a bioreactor. The whole-genomesequencing of the WTF-156T strain revealed eleven mutations. While no mutation was found amongthe genes encoding formate hydrogen lyase, a point mutation (G154A) was identified in a formatetransporter (TON_1573). The TON_1573 (A52T) mutation, when introduced into the parent strain,conferred increase in formate consumption and H2 production. Another adaptive passage, carried outby culturing repeatedly in a bioreactor, resulted in a strain, which has a mutation in TON_1573 (C155A) causing amino acid change, A52E. These results implicate that substitution of A52 residue of a formate transporter might be a critical factor to ensure the increase in formate uptake and cell growth.motic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecularchanges. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H2 production and formate consumption increased remarkably. The 156 times transferred-strain, WTF-156T, was demonstrated to enhance H2 production using formate in a bioreactor. The whole-genomesequencing of the WTF-156T strain revealed eleven mutations. While no mutation was found amongthe genes encoding formate hydrogen lyase, a point mutation (G154A) was identified in a formatetransporter (TON_1573). The TON_1573 (A52T) mutation, when introduced into the parent strain,conferred increase in formate consumption and H2 production. Another adaptive passage, carried outby culturing repeatedly in a bioreactor, resulted in a strain, which has a mutation in TON_1573 (C155A) causing amino acid change, A52E. These results implicate that substitution of A52 residue of a formate transporter might be a critical factor to ensure the increase in formate uptake and cell growth. -
dc.description.uri 2 -
dc.language English -
dc.publisher 미생물다양성생태워크샵 -
dc.relation.isPartOf 2017 미생물 다양성 생태 워크샵 -
dc.title Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 213 -
dc.citation.startPage 213 -
dc.citation.title 2017 미생물 다양성 생태 워크샵 -
dc.contributor.alternativeName 정해창 -
dc.contributor.alternativeName 이성혁 -
dc.contributor.alternativeName 이성목 -
dc.contributor.alternativeName 안영준 -
dc.contributor.alternativeName 이정현 -
dc.contributor.alternativeName 이현숙 -
dc.contributor.alternativeName 강성균 -
dc.identifier.bibliographicCitation 2017 미생물 다양성 생태 워크샵, pp.213 -
dc.description.journalClass 2 -
Appears in Collections:
Marine Resources Research Division > Marine Biotechnology Research Center > 2. Conference Papers
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