Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate

Title
Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate
Author(s)
정해창; 이성혁; 이성목; 안영준; 이정현; 이현숙; 강성균
KIOST Author(s)
Jung, Hae Chang(정해창)Lee, Seong Hyuk(이성혁)Young, Jun An(안영준)Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Alternative Author(s)
정해창; 이성혁; 이성목; 안영준; 이정현; 이현숙; 강성균
Publication Year
2017-08-24
Abstract
Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could growon formate and produce H2. Formate conversion to hydrogen was mediated by a formate-hydrogenlyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecularchanges. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H2 production and formate consumption increased remarkably. The 156 times transferred-strain, WTF-156T, was demonstrated to enhance H2 production using formate in a bioreactor. The whole-genomesequencing of the WTF-156T strain revealed eleven mutations. While no mutation was found amongthe genes encoding formate hydrogen lyase, a point mutation (G154A) was identified in a formatetransporter (TON_1573). The TON_1573 (A52T) mutation, when introduced into the parent strain,conferred increase in formate consumption and H2 production. Another adaptive passage, carried outby culturing repeatedly in a bioreactor, resulted in a strain, which has a mutation in TON_1573 (C155A) causing amino acid change, A52E. These results implicate that substitution of A52 residue of a formate transporter might be a critical factor to ensure the increase in formate uptake and cell growth.motic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecularchanges. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H2 production and formate consumption increased remarkably. The 156 times transferred-strain, WTF-156T, was demonstrated to enhance H2 production using formate in a bioreactor. The whole-genomesequencing of the WTF-156T strain revealed eleven mutations. While no mutation was found amongthe genes encoding formate hydrogen lyase, a point mutation (G154A) was identified in a formatetransporter (TON_1573). The TON_1573 (A52T) mutation, when introduced into the parent strain,conferred increase in formate consumption and H2 production. Another adaptive passage, carried outby culturing repeatedly in a bioreactor, resulted in a strain, which has a mutation in TON_1573 (C155A) causing amino acid change, A52E. These results implicate that substitution of A52 residue of a formate transporter might be a critical factor to ensure the increase in formate uptake and cell growth.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/23867
Bibliographic Citation
2017 미생물 다양성 생태 워크샵, pp.213, 2017
Publisher
미생물다양성생태워크샵
Type
Conference
Language
English
Publisher
미생물다양성생태워크샵
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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