Hypoxia-induced fibroblast growth factor 11 stimulates capillary-like endothelial tube formation SCIE SCOPUS

Cited 15 time in WEB OF SCIENCE Cited 15 time in Scopus
Title
Hypoxia-induced fibroblast growth factor 11 stimulates capillary-like endothelial tube formation
Author(s)
Yang, Jimin; Kim, Woo Jean; Jun, Hyoung Oh; Lee, Eun Ju; Lee, Kyeong Won; Jeong, Jae-Yeon; Lee, Sae-Won
KIOST Author(s)
Lee, Kyeong Won(이경원)
Publication Year
2015-11
Abstract
Low oxygen or hypoxia can be observed in the central region of solid tumors. Hypoxia is a strong stimulus for new blood vessel formation or angiogenesis, which is essential for tumor growth and progression. Fibroblast growth factor 11 (FGF11) is an intracellular non-secretory FGF whose function has not yet been fully characterized. In the present study, we demonstrated that FGF11 expression is upregulated under hypoxic conditions in human umbilical vein endothelial cells (HUVECs). FGF11 overexpression stimulated capillary-like tube formation, yet did not affect cell migration. Notably, FGF11 markedly increased the levels of tight junction proteins including occludin, zonula occludens-1 (ZO-1) and claudin-5 in HUVECs. The FGF11 promoter contains hypoxia response elements (HREs), and hypoxia-inducible factor-1 (HIF-1) binds to HREs to activate hypoxia-related genes. We demonstrated that hypoxia or HIF-1 expression under normoxic conditions increased the luciferase activity driven by the FGF11 promoter. However, deletion of the HREs from the FGF11 promoter rendered reporter gene activity unresponsive to hypoxia or HIF-1. Taken together, we propose that FGF11 may be involved in the stabilization of capillary-like tube structures associated with angiogenesis and may act as a modulator of hypoxia-induced pathological processes such as tumorigenesis.
ISSN
1021-335X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2380
DOI
10.3892/or.2015.4223
Bibliographic Citation
ONCOLOGY REPORTS, v.34, no.5, pp.2745 - 2751, 2015
Publisher
SPANDIDOS PUBL LTD
Subject
CLAUDIN 5 EXPRESSION; ANGIOGENESIS; PROTEIN; DIFFERENTIATION; MODULATION; MECHANISMS; APOPTOSIS; CELLS; VEGF
Keywords
tumor angiogenesis; hypoxia-inducible factor-1; hypoxia response element; growth factor; transcription factor; fibroblast growth factor 11
Type
Article
Language
English
Document Type
Article
Publisher
SPANDIDOS PUBL LTD
Related Researcher
Research Interests

molecular biology,cell biology,분자생물학,세포생물학

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