Transcriptomic responses to different concentrations of Fluoxetine in Hydra

Abstract

Depressive disorder became leading factor for mental and physical illness. Fluoxetine is a mostly prescribed drug to treat major symptoms of depression. We demonstrated acute toxicity test for Fluoxetine in Hydra magnipapillata after 24, 48 and 72 h of exposure. The median lethal concentrations (LC50) of Fluoxetine in H. magnipapillata were 3.678±0.324, 3.082±0.356 and 2.901±0.344 mg/L for 24, 48 and 72 h, respectively. Transcriptomic analysis was performed in H. magnipapillata at low concentration (30 μg/L FLX30) and high concentration (300 μg/L FLX300) for 12, 24, 48 and 72 h. The most strongly up- or downregulated genes (>5 fold) for FLX30 were Ferritin, Embryonic-1, Periculin, APX1, GSTM2, C21ORF29, CYP17A1, DAZL, CDK5RAP1, and NYC. These genes were involved in acute phase response, hemoglobin production, antioxidant protection, sexual maturation and cell differentiation. At FLX300, the most affected genes were TFIIIA, MUC2, PMS1 and SETMAR. These genes were involved in mucus development, sexual maturation and DNA repair. Genes associated with tumor progression and sex determination was also affected. These results extend our understanding for possible effect of Fluoxetine to aquatic organisms. and 72 h of exposure. The median lethal concentrations (LC50) of Fluoxetine in H. magnipapillata were 3.678±0.324, 3.082±0.356 and 2.901±0.344 mg/L for 24, 48 and 72 h, respectively. Transcriptomic analysis was performed in H. magnipapillata at low concentration (30 μg/L FLX30) and high concentration (300 μg/L FLX300) for 12, 24, 48 and 72 h. The most strongly up- or downregulated genes (>5 fold) for FLX30 were Ferritin, Embryonic-1, Periculin, APX1, GSTM2, C21ORF29, CYP17A1, DAZL, CDK5RAP1, and NYC. These genes were involved in acute phase response, hemoglobin production, antioxidant protection, sexual maturation and cell differentiation. At FLX300, the most affected genes were TFIIIA, MUC2, PMS1 and SETMAR. These genes were involved in mucus development, sexual maturation and DNA repair. Genes associated with tumor progression and sex determination was also affected. These results extend our understanding for possible effect of Fluoxetine to aquatic organisms.