Transcriptomic analysis of Hydra magnipapillata exposed to low and high concentrations of antidepressant
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ade Yamindago | - |
dc.contributor.author | 염승식 | - |
dc.date.accessioned | 2020-07-15T13:34:02Z | - |
dc.date.available | 2020-07-15T13:34:02Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2017-11-07 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/23607 | - |
dc.description.abstract | Antidepressant drug Fluoxetine is popularly used to treat human major depressive symptoms. This drug is potentially impact to aquatic organisms. Acute toxicity of Fluoxetine was investigated in a fresh water cnidarian, H. magnipapillata. Median lethal concentration (LC50) values were 3.7 (24 h), 3.1 (48 h) and 2.9 mg/L (72 h). At molecular level, transcriptomic analysis was also performed in H. magnipapillata after exposure of Fluoxetine for 12, 24, 48 and 72 h at environmentally low (30 μg/L) and high (300 μg/L) concentrations. The analysis revealed that the genes whose expressions up- or down-regulated (≥ 1.5 fold) were highly dynamic after exposure of Fluoxetine at low concentration (560 genes) compared to high concentration (430 genes). Functional annotation analysis of the differentially expressed genes revealed chitin metabolic process, aminoglycan metabolic process, and polysaccharide metabolic process and nitrogen metabolism in the exposed H. magnipapillata. Genes associated with CREB signaling, tumor progression, sex determination, steroid and response to stress as well as iron regulation was also affected by Fluoxetine. Changes in morphology indicated as reduction of size of tentacles and body in the exposed H. magnipapillata were also observed suggesting possible role of GABAergic neurotransmitter system.dian lethal concentration (LC50) values were 3.7 (24 h), 3.1 (48 h) and 2.9 mg/L (72 h). At molecular level, transcriptomic analysis was also performed in H. magnipapillata after exposure of Fluoxetine for 12, 24, 48 and 72 h at environmentally low (30 μg/L) and high (300 μg/L) concentrations. The analysis revealed that the genes whose expressions up- or down-regulated (≥ 1.5 fold) were highly dynamic after exposure of Fluoxetine at low concentration (560 genes) compared to high concentration (430 genes). Functional annotation analysis of the differentially expressed genes revealed chitin metabolic process, aminoglycan metabolic process, and polysaccharide metabolic process and nitrogen metabolism in the exposed H. magnipapillata. Genes associated with CREB signaling, tumor progression, sex determination, steroid and response to stress as well as iron regulation was also affected by Fluoxetine. Changes in morphology indicated as reduction of size of tentacles and body in the exposed H. magnipapillata were also observed suggesting possible role of GABAergic neurotransmitter system. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ASEAN-FEN | - |
dc.relation.isPartOf | ASEAN-FEN International Fisheries Symposium 2017 | - |
dc.title | Transcriptomic analysis of Hydra magnipapillata exposed to low and high concentrations of antidepressant | - |
dc.type | Conference | - |
dc.citation.endPage | 477 | - |
dc.citation.startPage | 477 | - |
dc.citation.title | ASEAN-FEN International Fisheries Symposium 2017 | - |
dc.contributor.alternativeName | Ade Yamindago | - |
dc.contributor.alternativeName | 염승식 | - |
dc.identifier.bibliographicCitation | ASEAN-FEN International Fisheries Symposium 2017, pp.477 | - |
dc.description.journalClass | 1 | - |