Saringosterol acetate of Hizikia fusiforme exhibits an anti-cancer effect on Hep3B and Du145 cell lines

Abstract

Hizikia fusiforme, an edible brown alga, is widely consumed in Korea, Japan, and China and possesses a number of potentially beneficial biological functionalities, including anti-oxidants, anti-coagulant, anti-cancer, and anti-inflammation. Therefore, the potent anti-cancer effects of saringostrol acetate (SSA) isolated from 70% EtOH extraction of H. fusiforme was investigated for its inhibitory effects on liver and prostate cancer in Hep3B and Du145 cell lines. The SSA markedly inhibited cancer cell growth and increased the population of cells in sub-G1 compared to the control. Furthermore, we confirmed that the mechanism involved in this process occurs through PI3K/Akt/mTOR pathway an important regulator of cell growth, metabolism, survival, metastasis, and resistance to chemotherapy. Therefore, SSA significantly reduced the expression of PI3K, Akt, mTOR protein levels in both cells. These results indicated that the SSA has the potential to be used in applications related with nutraceuticals or functional foods to reduce carcinogenic effects. Therefore, the potent anti-cancer effects of saringostrol acetate (SSA) isolated from 70% EtOH extraction of H. fusiforme was investigated for its inhibitory effects on liver and prostate cancer in Hep3B and Du145 cell lines. The SSA markedly inhibited cancer cell growth and increased the population of cells in sub-G1 compared to the control. Furthermore, we confirmed that the mechanism involved in this process occurs through PI3K/Akt/mTOR pathway an important regulator of cell growth, metabolism, survival, metastasis, and resistance to chemotherapy. Therefore, SSA significantly reduced the expression of PI3K, Akt, mTOR protein levels in both cells. These results indicated that the SSA has the potential to be used in applications related with nutraceuticals or functional foods to reduce carcinogenic effects.