Fabrication of Poly(vinyl alcohol)hydrogel blended with red sea cucumber for wound treatment

Abstract

When skin wound occur, hydrogel patches have been generally used as wound dressing for wound healing. Poly (vinyl alcohol) (PVA) is one of the commonplace hydrogel patch’s materials which have non-toxicity, non-carcinogenicity, biocompatibility and easy processing. Red sea cucumber (RSC) is widely found in Jeju Island in Korea and It has cell proliferation on neonatal normal human dermal fibroblast (NHDF). So, we designed to blend PVA hydrogel patches with RSC and fabricated without chemical crosslink by using freezing/thawing method to make more effective PVA hydrogel-patches on wound healing. Then, we evaluated swelling property in water and mechanical property to know hydrogel patch’s mechanical characterization and intermolecular interactions of hydrogel affected by RSC were elucidated using FTIR and cell proliferation was measured by MTT assay. As a result, Hydrogel patches blended with RSC observed significant increase of cell growth without cytotoxicity. In addition, hydrogel indicated a high water absorption and mechanical properties are suitable for wound dressing. Although further studies, including in vivo and signalling pathway for wound healing, will be studied, thecomprehensive results suggest that RSC hydrogel patches are promising candidates for wound dressing and tissue engineering applications.ility and easy processing. Red sea cucumber (RSC) is widely found in Jeju Island in Korea and It has cell proliferation on neonatal normal human dermal fibroblast (NHDF). So, we designed to blend PVA hydrogel patches with RSC and fabricated without chemical crosslink by using freezing/thawing method to make more effective PVA hydrogel-patches on wound healing. Then, we evaluated swelling property in water and mechanical property to know hydrogel patch’s mechanical characterization and intermolecular interactions of hydrogel affected by RSC were elucidated using FTIR and cell proliferation was measured by MTT assay. As a result, Hydrogel patches blended with RSC observed significant increase of cell growth without cytotoxicity. In addition, hydrogel indicated a high water absorption and mechanical properties are suitable for wound dressing. Although further studies, including in vivo and signalling pathway for wound healing, will be studied, thecomprehensive results suggest that RSC hydrogel patches are promising candidates for wound dressing and tissue engineering applications.