Gene expression changes associated with dose-response of Fluoxetine in Hydra

Abstract

Fluoxetine is a selective serotonin reuptake inhibitors (SSRIs) drug to treat human behavioral disorders. We investigated effects of Fluoxetine in Hydra magnipapillata to predict biological responses of aquatic organism to this drug. The median lethal concentrations (LC50) of Fluoxetine in H. magnipapillata were 3.678±0.324 (24 h), 3.082±0.356 (48 h) and 2.901±0.344 mg/L (72 h). We evaluated transcriptomic gene changes associated with low (30 μg/L, FLX30) and high (300 μg/L, FLX300) doses of Fluoxetine in H. magnipapillata for 12, 24, 48 and 72 h. The differentially expressed genes of both FLX30 and FLX300 groups were mainly associated with cellular process and signaling. The genes involved in DNA repair, tumor suppression/progression, cell death, stress response, acute phase response, inflammatory response, respiratory gaseous exchange, development and growth, sex determination, sexual reproduction, nucleotide metabolism and blood organelle were also affected in both groups. Genes associated with locomotory behavior, insulin resistance and response to drug were specifically altered in FLX30 group, and genes associated with cell adhesion, vesicle fusion and visual perception were specifically altered in FLX300 group. Thus, our study suggests insight into variation of doses associated with molecular responses of aquatic organisms exposed to Fluoxetine.dian lethal concentrations (LC50) of Fluoxetine in H. magnipapillata were 3.678±0.324 (24 h), 3.082±0.356 (48 h) and 2.901±0.344 mg/L (72 h). We evaluated transcriptomic gene changes associated with low (30 μg/L, FLX30) and high (300 μg/L, FLX300) doses of Fluoxetine in H. magnipapillata for 12, 24, 48 and 72 h. The differentially expressed genes of both FLX30 and FLX300 groups were mainly associated with cellular process and signaling. The genes involved in DNA repair, tumor suppression/progression, cell death, stress response, acute phase response, inflammatory response, respiratory gaseous exchange, development and growth, sex determination, sexual reproduction, nucleotide metabolism and blood organelle were also affected in both groups. Genes associated with locomotory behavior, insulin resistance and response to drug were specifically altered in FLX30 group, and genes associated with cell adhesion, vesicle fusion and visual perception were specifically altered in FLX300 group. Thus, our study suggests insight into variation of doses associated with molecular responses of aquatic organisms exposed to Fluoxetine.