Anti-liver cancer effect of saringosterol acetate isolated from hizikia fusiforme in diethylnitrosamin-induced zebrafish embryo

Abstract

Diethylnitrosamin (DEN) is a well-known potent hepatocarcinogenic agent present in tobacco smoke, water, cured and fried meals, cheddar cheese,agricultural chemicals, cosmetics and pharmaceutical products. DEN is known to induce damage on many enzymes involved in DNA repair and isnormally used to induce liver cancer in experimental animal models. Therefore, this study has confirmed an increase in the production of reactiveoxygen species (ROS) and induction of cell death by DEN treatment. And angiogenesis is a necessary condition for invasion and metastasisthrough angiogenic factors (VEGF, TFGβ1, MMP2). These factors are also increased by DEN treatment in zebrafish. We observed that exposure tosaringostrol acetate (SSA), significantly inhibited DEN induced ROS, cell death and angiogenic factors. These finding indicate that zebrafish modelis an efficient animal model that can be used to investigate DEN-stimulated cancer. Therefore, this model can be used as an in vivo experiment toconfirm the anticancer properties of functional foods and nutraceuticals many enzymes involved in DNA repair and isnormally used to induce liver cancer in experimental animal models. Therefore, this study has confirmed an increase in the production of reactiveoxygen species (ROS) and induction of cell death by DEN treatment. And angiogenesis is a necessary condition for invasion and metastasisthrough angiogenic factors (VEGF, TFGβ1, MMP2). These factors are also increased by DEN treatment in zebrafish. We observed that exposure tosaringostrol acetate (SSA), significantly inhibited DEN induced ROS, cell death and angiogenic factors. These finding indicate that zebrafish modelis an efficient animal model that can be used to investigate DEN-stimulated cancer. Therefore, this model can be used as an in vivo experiment toconfirm the anticancer properties of functional foods and nutraceuticals