Inhibitors of RANKL-induced osteoclast differentiation from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.

Title
Inhibitors of RANKL-induced osteoclast differentiation from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.
Author(s)
최병규; 이화선; 이희승; 이종석; 이연주; 이지훈; 신희재
KIOST Author(s)
Choi, Byeoung Kyu(최병규)Lee, Hwa Sun(이화선)Lee, Hyi Seung(이희승)Lee, Jong Seok(이종석)Lee, Yeon Ju(이연주)Lee, Jihoon(이지훈)
Publication Year
2018-07-03
Abstract
Large numbers of novel secondary metabolites from the ocean have structural diversities and potent bioactivities. Many marine organisms, especially microorganisms, have been attracting significant attention from natural product chemists and biologists due to the potential of marine natural products as drugs during recent years. As part of our continuing efforts to discover bioactive natural products from sponge-derived microorganisms, various marine sponges were collected and investigated for the isolation and identification of microorganisms. During studies on the diversity of fungi, a marine fungal strain 01NT-1.1.5, Aspergillus flocculosus derived from a sponge Stylissa sp., was isolated through experimental tools for the isolation of microorganisms. The crude extract of the strain was purified further by a reversed-phase HPLC to yield one new compound, ochraceopone F (1), and four known compounds aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5). The structures of the known compounds were identified by 1D and 2D NMR analysis and comparison with literature data. All compounds were tested for anti-proliferative activity on human cancer cell lines and RANKL-induced osteoclast differentiation inhibitory effect using a TRAP assay. Among compounds 1-5, mactanamide (5) showed potent RANKL-induced osteoclast differentiation inhibitory effect. biologists due to the potential of marine natural products as drugs during recent years. As part of our continuing efforts to discover bioactive natural products from sponge-derived microorganisms, various marine sponges were collected and investigated for the isolation and identification of microorganisms. During studies on the diversity of fungi, a marine fungal strain 01NT-1.1.5, Aspergillus flocculosus derived from a sponge Stylissa sp., was isolated through experimental tools for the isolation of microorganisms. The crude extract of the strain was purified further by a reversed-phase HPLC to yield one new compound, ochraceopone F (1), and four known compounds aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5). The structures of the known compounds were identified by 1D and 2D NMR analysis and comparison with literature data. All compounds were tested for anti-proliferative activity on human cancer cell lines and RANKL-induced osteoclast differentiation inhibitory effect using a TRAP assay. Among compounds 1-5, mactanamide (5) showed potent RANKL-induced osteoclast differentiation inhibitory effect.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/23194
Bibliographic Citation
18th European Congress On Biotechnology, pp.82, 2018
Publisher
The European Federation of Biotechnology
Type
Conference
Language
English
Publisher
The European Federation of Biotechnology
Related Researcher
Research Interests

Marine Natural Products,Organic Chemistry,해양천연물,유기화학

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