Improvement of 2,3-butanediol production from CO gas by random mutagenesis of Clostridium sp. AWRP
DC Field | Value | Language |
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dc.contributor.author | 권수재 | - |
dc.contributor.author | 이종민 | - |
dc.contributor.author | 강성균 | - |
dc.contributor.author | 이현숙 | - |
dc.date.accessioned | 2020-07-15T11:33:46Z | - |
dc.date.available | 2020-07-15T11:33:46Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2018-07-04 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/23190 | - |
dc.description.abstract | Some acetogenic bacteria has the ability to use carbon monoxide (CO) using the Wood-Ljungdahl pathway. Biological conversion of CO using acetogens offers benefits of utilizing industrial waste gases containing CO and generating valuable fuels such as ethanol, butanol and 2,3-butanediol. In this study, we screened 2,3-butanediol-producing microbes among various environmental samples and isolated a new strain, Clostridium sp. AWRP from a wetland. This strain is able to produce acetate, ethanol and 2,3-butanediol as major metabolites using CO. To improve 2,3-butanediol production, the wild-type strain was modified by random mutagenesis technology. After treatment of cells with N-methyl-N-nitro-N-nitrosoguanidine (NTG), mutants with better growth than the wild-type strain under high CO partial pressure were selected. They showed different growth and metabolite production patterns. Among them, a few mutants were identified to have improved CO consumption and 2,3-butanediol production. Here, we describe effective strategies for selection of CO-utilizing 2,3-butanediol producers through enrichment processes during isolation and mutagenesis.els such as ethanol, butanol and 2,3-butanediol. In this study, we screened 2,3-butanediol-producing microbes among various environmental samples and isolated a new strain, Clostridium sp. AWRP from a wetland. This strain is able to produce acetate, ethanol and 2,3-butanediol as major metabolites using CO. To improve 2,3-butanediol production, the wild-type strain was modified by random mutagenesis technology. After treatment of cells with N-methyl-N-nitro-N-nitrosoguanidine (NTG), mutants with better growth than the wild-type strain under high CO partial pressure were selected. They showed different growth and metabolite production patterns. Among them, a few mutants were identified to have improved CO consumption and 2,3-butanediol production. Here, we describe effective strategies for selection of CO-utilizing 2,3-butanediol producers through enrichment processes during isolation and mutagenesis. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국화학공학회 | - |
dc.relation.isPartOf | C1 가스 리파이너리 심포지엄 | - |
dc.title | Improvement of 2,3-butanediol production from CO gas by random mutagenesis of Clostridium sp. AWRP | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.title | C1 가스 리파이너리 심포지엄 | - |
dc.contributor.alternativeName | 권수재 | - |
dc.contributor.alternativeName | 이종민 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.identifier.bibliographicCitation | C1 가스 리파이너리 심포지엄 | - |
dc.description.journalClass | 2 | - |